CAJ | 학술논문

目的观察糖尿病豚鼠膀胱Cajal样间质细胞形态学变化,探讨糖尿病膀胱功能改变原因.方法用链脲佐菌素单次注射建立豚鼠糖尿病模型,建模成功4周后,行尿动力学检查,采用组织冷冻切片、酪氨酸蛋白激酶受体单克隆抗体间接免疫荧光检测及透射电镜技术观察膀胱中Cajal样间质细胞变化.结果与对照组相比,糖尿病豚鼠膀胱最大逼尿肌压显著降低,最大膀胱容量及膀胱顺应性显著明显增高.免疫荧光检测显示逼尿肌中KIT阳性细胞与逼尿肌组织面积百分比均明显小于对照组.电镜下可见Cajal样间质细胞内细胞器变性、减少,胞质广泛溶解,核周间隙增宽,与其他细胞间的缝隙连接显著减少、结构破坏、连接松散.结论糖尿病豚鼠膀胱Cajal样间质细胞的数量减少和结构破坏可能是造成膀胱功能障碍的重要原因之一.
목적 관찰당뇨병돈서방광Cajal양간질세포형태학변화,탐토당뇨병방광공능개변원인.방법 용련뇨좌균소단차주사건립돈서당뇨병모형,건모성공4주후,행뇨동역학검사,채용조직냉동절편、락안산단백격매수체단극륭항체간접면역형광검측급투사전경기술관찰방광중Cajal양간질세포변화.결과 여대조조상비,당뇨병돈서방광최대핍뇨기압현저강저,최대방광용량급방광순응성현저명현증고.면역형광검측현시핍뇨기중KIT양성세포여핍뇨기조직면적백분비균명현소우대조조.전경하가견Cajal양간질세포내세포기변성、감소,포질엄범용해,핵주간극증관,여기타세포간적봉극련접현저감소、결구파배、련접송산.결론 당뇨병돈서방광Cajal양간질세포적수량감소화결구파배가능시조성방광공능장애적중요원인지일.
Purpose To clarify the morphologic changes and significance of interstitial cells of Cajal-like cells in the bladder of diabetic guinea pig.Methods The experimental guinea pigs were induced by a single intraperitoneal injection of streptozotocin(STZ).The diabetic guinea pig model, who were made succesful for 4 weeks, were detected through the urodynamic evaluation.The distribution of ICCs-like cells in the detrusor, which were taken from these guinea pigs,were quantitatively analyzed after frozen section and indirect immunofluorescence methods and observed by electron microscopy.Results In the diabetic group, the maximum detrusor pressure was signifently lower than controls (P＜0.01), maximum bladder capacity, compliance, residual volume and leak point pressures was significently higer than controls (P＜0.05 or P＜0.01);In detrusor, compared with control, the ICCs-like cells of diabetes group showed significently decreased in quantity. In diabetic bladder, the quantity of gap junctions between ICCs-like cells and cells around,such as smoth muscle cells,terminal nerves and other ICCs-like cells,decreased significently.And more cavities and less organoids occurred in cytoplasm of diabetic ICCs-like cells. Mitochondria became swollen or even disapeared, accompanied with swollen endocytoplasmic reticulum.Conclusion The decrease and injures of ICCs-like cells in bladder may be one of the mechanisms resulting in urodynamic changes of diabetic cystopathy.