CAJ | 학술논문

基底材料的拓扑形貌是影响细胞行为的重要因素之一,材料表面微纳米图案化不但可以提供规则的结构模版,用以研究细胞对生长环境的响应特性,而且可以为组织再生用支架和植入性器件的设计提供基础数据.以表面具有微纳米沟槽结构的聚氨酯薄膜为基底材料,选择在促进组织修复和再生中起重要作用的成纤维细胞为模型细胞,通过细胞活性检测和免疫荧光分析,探讨材料表面的微纳米图案结构对成纤维细胞黏附、增殖、形态以及细胞骨架发育的作用.实验结果表明,微纳米沟槽结构能够明显促进成纤维细胞在材料表面的黏附和增殖,并诱导细胞响应所生长的微纳米沟槽结构进行骨架重排.
기저재료적탁복형모시영향세포행위적중요인소지일,재료표면미납미도안화불단가이제공규칙적결구모판,용이연구세포대생장배경적향응특성,이차가이위조직재생용지가화식입성기건적설계제공기출수거.이표면구유미납미구조결구적취안지박막위기저재료,선택재촉진조직수복화재생중기중요작용적성섬유세포위모형세포,통과세포활성검측화면역형광분석,탐토재료표면적미납미도안결구대성섬유세포점부、증식、형태이급세포골가발육적작용.실험결과표명,미납미구조결구능구명현촉진성섬유세포재재료표면적점부화증식,병유도세포향응소생장적미납미구조결구진행골가중배.
Surface topography is one of the potential important factors for biomaterials, which influences cells behaviors such as cell adhesion, proliferation, differentiation and even apoptosis.It is clear that cells would respond to nanometric scale surface features, therefore it is possible to induce certain responses of cells by fabricating substrates with combined micro-nano patterns.Herein, transparent polyurethane films with micro-nano groove patterns were prepared by solvent-casting from a template.Mouse fibroblast cells (3T3-L1) were seeded on the patterning films and cultivated for at least one week.Cell morphologies and cytoskeleton protein expression in the fibroblasts were observed with optical microscope and confocal laser scanning microscopy.Cells adhesion and proliferation were examined using MTS assay.Experimental results showed that the micro-nano groove pattern significantly enhanced the adhesion and proliferation of fibroblasts.The micro-nano groove pattern also showed effects in inducing cytoskeleton rearrangement as well as promoting actin and tubulin expression.