CAJ | 학술논문

采用PCR-RFLP方法,对2008年引进的SPF凡纳滨对虾群体(G0)与2006年引进亲虾繁育的两代群体(G1、G2)的遗传结构进行了分析.使用Alu I、Taq I、Mbo I、Spe I、Ssp I 共5种限制性内切酶对线粒体DNA控制区进行单酶切分析,酶切得到的单倍型在2～4个之间,三个群体的复合单倍型类型分别有3个、7个和9个,没有为三个群体所共有的复合单倍型,复合单倍型的分布显示群体间差异显著.三个群体(G0、G1、G2)的核苷酸多样度π值逐渐增加,分别为0.033 3、0.128 6、0.134 9,复合单倍型多样度h分别为0.622 2、0.850 0、0.847 0,说明养殖群体的遗传多样性大于引进群体.聚类分析的结果表明随着养殖世代的增加,养殖群体与引进群体的差异呈增大的趋势.由此推测G0与G1的亲本存在较大的遗传差异,并且在使用引进群体作为亲本的育种过程中可能出现了种质混杂.
채용PCR-RFLP방법,대2008년인진적SPF범납빈대하군체(G0)여2006년인진친하번육적량대군체(G1、G2)적유전결구진행료분석.사용Alu I、Taq I、Mbo I、Spe I、Ssp I 공5충한제성내절매대선립체DNA공제구진행단매절분석,매절득도적단배형재2～4개지간,삼개군체적복합단배형류형분별유3개、7개화9개,몰유위삼개군체소공유적복합단배형,복합단배형적분포현시군체간차이현저.삼개군체(G0、G1、G2)적핵감산다양도π치축점증가,분별위0.033 3、0.128 6、0.134 9,복합단배형다양도h분별위0.622 2、0.850 0、0.847 0,설명양식군체적유전다양성대우인진군체.취류분석적결과표명수착양식세대적증가,양식군체여인진군체적차이정증대적추세.유차추측G0여G1적친본존재교대적유전차이,병차재사용인진군체작위친본적육충과정중가능출현료충질혼잡.
In this study,genetic structures of three populations were analysed,including population G0 imported from abroad in 2008 and two cultured populations(G1 and G2) of filial generations bred from imported primary parents in 2006.Five restriction enzymes(Alu I、Taq I、Mbo I、Spe I、Ssp I) were used in the PCR-RFLP analysis of the mtDNA control region.The number of haplotype was between two and four.There were 3、7 and 9 kinds of composite haplotypes in the three populations respectively.No composite haplotype shared by three populations was detected, and the distribution of the composite haplotypes demonstrated that there was a significant difference.The π value of the three populations(G0、G1、G2)increased gradually, being 0.033 3、0.128 6 and 0.134 9 respectively. Composite haplotype diversity(h) of the three populations were 0.622 2、0.850 0 and 0.847 0 respectively ,which indicated that the cultured stocks had a higher genetic diversity compared with the imported stock. The dendrogram showed that there was an increasing genetic difference between the cultured stocks and imported stock as generation proceeded. Therefore, it was presumed that there be major differences between G0 and the imported primary parents of G1, and population mixing might have happened when the imported population was employed as primary parents in the breeding programme.