CAJ | 학술논문

用垂直凝胶电泳来研究采自中国的(条斑紫菜×坛紫菜)杂交种、坛紫菜、条斑紫菜、少精紫菜、半叶紫菜的同工酶多态性.然后分析最小可能位点数和观察同位基因数、遗传差异性和采用平均连接聚类法.选取23种酶带中的六个酶(MDH,ME,LDH,GDH,IDH和 G-6-PDH)进行分析.最小可能等位基因位点数显示五种紫菜在ME等位基因位点都只有一个.LDH 和GDH 等位基因位点半叶紫菜和少精紫菜和另外的三种紫菜不同,而在分析MDH时半叶紫菜比较独特.在IDH位点分析时少精紫菜和坛紫菜和其他三种不同,而条斑紫菜和坛紫菜和其他三种不同在G-6-PDH位点不一致.总的来看,最小可能等位基因位点数分析半叶紫菜是最分离的.遗传多样性结果分析表明当遗传相似度为0.755 0时,五种紫菜中的遗传变异研究受到限制.条斑紫菜和坛紫菜的杂交种非常接近少精紫菜.当遗传相似度达到0.893 7时,出乎意料的是条斑紫菜和坛紫菜遗传同一性很低.条斑紫菜4个株系的平均遗传相似度仅为0.742 8,差异比较大.在所有紫菜中半叶紫菜是最分化的分支,它与最小可能等位基因位点数分析一致.
용수직응효전영래연구채자중국적(조반자채×단자채)잡교충、단자채、조반자채、소정자채、반협자채적동공매다태성.연후분석최소가능위점수화관찰동위기인수、유전차이성화채용평균련접취류법.선취23충매대중적륙개매(MDH,ME,LDH,GDH,IDH화 G-6-PDH)진행분석.최소가능등위기인위점수현시오충자채재ME등위기인위점도지유일개.LDH 화GDH 등위기인위점반협자채화소정자채화령외적삼충자채불동,이재분석MDH시반협자채비교독특.재IDH위점분석시소정자채화단자채화기타삼충불동,이조반자채화단자채화기타삼충불동재G-6-PDH위점불일치.총적래간,최소가능등위기인위점수분석반협자채시최분리적.유전다양성결과분석표명당유전상사도위0.755 0시,오충자채중적유전변이연구수도한제.조반자채화단자채적잡교충비상접근소정자채.당유전상사도체도0.893 7시,출호의료적시조반자채화단자채유전동일성흔저.조반자채4개주계적평균유전상사도부위0.742 8,차이비교대.재소유자채중반협자채시최분화적분지,타여최소가능등위기인위점수분석일치.
Vertical polyamide gel electrophoresis was used to investigate isozyme polymorphisms among different isolates (including wild and cultivated) of Porphyra katadai, Porphyra oligospermatangia, Porphyra yezoensis, Porphyra haitanensis, and a hybridize species (Porphyra yezoensis × Porphyra haitanensis) sampled from China. Whereafter, the analyses of probable minimum loci numbers, observed alleles sum, genetic diversity, and unweighted pair-group arithmetic average (UPGMA) cluster were carried out. After initial activity and resolution testing of bands of 23 enzymes, 6 of them (MDH, ME, LDH, GDH, IDH and G-6-PDH) were proved to be appropriate for analysis of the full sample set. The probable minimum numbers of loci and alleles analyses showed that the five species of Porphyra had an extraordinary consistent result in ME loci and alleles. However, P. katadai and P. oligospermatangia differed from other three species of Porphyra in LDH and GDH loci and alleles. P. katadai was independent in the analyses of MDH and P. oligospermatangia and P. haitanensis differed from other three species in IDH analyses. Moreover, P. yezoensis and P. haitanensis were apart from other three species in G-6-PDH analysis. Taking one with another, P. katadai was relatively separated in the probable minimum numbers of loci and alleles analyses. The results indicated that the genetic variation among the five Porphyra species was limited with a genetic identity of 0.7550. The hybridize species (P. yezoensis × P. haitanensis) seemed to be high homologue with P. oligospermatangia, unexpectedly got relatively lower average genetic identities with both P. yezoensis and P. haitanensis. The 4 strains of P. yezoensis were relatively divergent with an average genetic identity of 0.7428, and P. katadai presented the most differentiated, compared with other species, which consistented with the result summarized in the probable minimum numbers of loci and alleles analyses.