中华眼外伤职业眼病杂志
中華眼外傷職業眼病雜誌
중화안외상직업안병잡지
CHINESE JOURNAL OF OCULAR TRAUMA AND OCCUPATIONAL EYE DISEASE
2011年
12期
881-884
,共4页
傅少颖%刘红玲%候勇生%高轶悝
傅少穎%劉紅玲%候勇生%高軼悝
부소영%류홍령%후용생%고질리
交感性眼炎%眼内异物%病理组织学
交感性眼炎%眼內異物%病理組織學
교감성안염%안내이물%병리조직학
Sympathetic ophthalmia%Intraocular foreign body%Histopathology
目的 观察大鼠单侧眼外伤能否诱发交感性眼炎,为交感性眼炎的发病机制提供理论依据.方法 将36只Wistar健康成年大鼠随机分为Ⅰ组眼内异物(砂粒)伤组、Ⅱ组异物(刀片)伤组、Ⅲ组正常对照组.正常对照组大鼠4只,不做任何处理.Ⅰ组、Ⅱ组中各16只大鼠右眼为异物植入眼,将不同异物经睫状体部位置入玻璃体腔,获得大鼠单眼异物伤型,通过额带放大镜观察双眼前段变化,并于2、4、6、8周摘除眼球,应用HE染色观察双眼球的组织病理学变化.结果 Ⅰ组、Ⅱ组大鼠右眼眼前段主要表现为球结膜充血、水肿,创口处有异常分泌物,眼球萎缩随时间延长例数增加.组织病理学可见中性粒细胞、淋巴细胞浸润.左眼无眼前段变化及组织病理学变化.Ⅲ组未见病理性改变.所有外伤眼均未能诱发交感性眼炎.结论 交感性眼炎的发病机制复杂,通过单纯模拟交感性眼炎的易发条件未能诱发出交感性眼炎.
目的 觀察大鼠單側眼外傷能否誘髮交感性眼炎,為交感性眼炎的髮病機製提供理論依據.方法 將36隻Wistar健康成年大鼠隨機分為Ⅰ組眼內異物(砂粒)傷組、Ⅱ組異物(刀片)傷組、Ⅲ組正常對照組.正常對照組大鼠4隻,不做任何處理.Ⅰ組、Ⅱ組中各16隻大鼠右眼為異物植入眼,將不同異物經睫狀體部位置入玻璃體腔,穫得大鼠單眼異物傷型,通過額帶放大鏡觀察雙眼前段變化,併于2、4、6、8週摘除眼毬,應用HE染色觀察雙眼毬的組織病理學變化.結果 Ⅰ組、Ⅱ組大鼠右眼眼前段主要錶現為毬結膜充血、水腫,創口處有異常分泌物,眼毬萎縮隨時間延長例數增加.組織病理學可見中性粒細胞、淋巴細胞浸潤.左眼無眼前段變化及組織病理學變化.Ⅲ組未見病理性改變.所有外傷眼均未能誘髮交感性眼炎.結論 交感性眼炎的髮病機製複雜,通過單純模擬交感性眼炎的易髮條件未能誘髮齣交感性眼炎.
목적 관찰대서단측안외상능부유발교감성안염,위교감성안염적발병궤제제공이론의거.방법 장36지Wistar건강성년대서수궤분위Ⅰ조안내이물(사립)상조、Ⅱ조이물(도편)상조、Ⅲ조정상대조조.정상대조조대서4지,불주임하처리.Ⅰ조、Ⅱ조중각16지대서우안위이물식입안,장불동이물경첩상체부위치입파리체강,획득대서단안이물상형,통과액대방대경관찰쌍안전단변화,병우2、4、6、8주적제안구,응용HE염색관찰쌍안구적조직병이학변화.결과 Ⅰ조、Ⅱ조대서우안안전단주요표현위구결막충혈、수종,창구처유이상분비물,안구위축수시간연장례수증가.조직병이학가견중성립세포、림파세포침윤.좌안무안전단변화급조직병이학변화.Ⅲ조미견병이성개변.소유외상안균미능유발교감성안염.결론 교감성안염적발병궤제복잡,통과단순모의교감성안염적역발조건미능유발출교감성안염.
Objective To investigate whether sympathetic ophthalmia could be induced by setting up the rat model of unilateral mechanical ocular trauma.And to discuss the mechanism of sympathetic ophthalmia.Methods 36 Wistar rats were randomly divided into three groups.In group Ⅰ,one piece of stone embeded in one eye (n =16) ; group Ⅱ,with a piece of blade embeded in one eye (n =16) ; groupⅢ,normal eye as control group (n =4).In group Ⅰ and group Ⅱ,intraocular foreign bodies were embeded from pars plana into the vitreous of the right eyes.All the eyes were observed with the loupe.The histopathological changes were observed under HE stain and microscopy 2 weeks,4 weeks,6 weeks and 8 weeks after the establishment of injury model.Results In group Ⅰ and group Ⅱ,abnormal secretions,the conjunctiva congest and oedema were main manifest in the right eyes.The number of atrophy of eyeballs increased with the time past.Histopathology showed lymphocytes and neutrophilic granulocytes infiltration in the eyeball.And abnormal changes were not found in group Ⅲ.It was failed to evocate the sympathetic ophthalmia in the injury models.Conclusion The pathogenesis of sympathetic ophthalmia is complex.Sympathetic ophthalmia were not evocated by setting up rat models of mechanical eye injury.
