CAJ | 학술논문

目的检测c-FLIP在胆囊癌组织中的表达,探讨siRNA(small interfering RNA)干扰胆囊癌细胞c-FLIP表达后对细胞生长的影响.方法用免疫组化法检测10例正常胆囊组织,10例胆囊腺瘤和35例胆囊癌组织中c-FLIP的表达;siRNA干扰c-FLIP表达后,通过生长曲线测定、倒置相差显微镜检查和流式细胞仪检测细胞生长状况.结果在胆囊癌组织中,c-FLIP的表达明显高于在胆囊腺瘤(P<0.05)和正常胆囊组织中的表达(P<0.05);siRNA干扰c-FLIP表达后,胆囊癌细胞株SGC-996的生长明显受到抑制,倒置相差显微镜检查发现,干扰后细胞凋亡细胞比例明显增加,流式细胞仪的检查发现干扰后细胞凋亡比例与对照组相比明显上升(P<0.05).结论 siRNA干扰c-FLIP表达的靶向治疗有可能成为治疗胆囊癌的新的方法.
목적 검측c-FLIP재담낭암조직중적표체,탐토siRNA(small interfering RNA)간우담낭암세포c-FLIP표체후대세포생장적영향.방법 용면역조화법검측10례정상담낭조직,10례담낭선류화35례담낭암조직중c-FLIP적표체;siRNA간우c-FLIP표체후,통과생장곡선측정、도치상차현미경검사화류식세포의검측세포생장상황.결과 재담낭암조직중,c-FLIP적표체명현고우재담낭선류(P<0.05)화정상담낭조직중적표체(P<0.05);siRNA간우c-FLIP표체후,담낭암세포주SGC-996적생장명현수도억제,도치상차현미경검사발현,간우후세포조망세포비례명현증가,류식세포의적검사발현간우후세포조망비례여대조조상비명현상승(P<0.05).결론 siRNA간우c-FLIP표체적파향치료유가능성위치료담낭암적신적방법.
Objective To investigate the expression of c-FLIP in gallbladder cancer and explore the effect of siRNA(small interfering RNA) to interfere expression of c-FLIP on gallbladder cancer cell line. Methods The expression of c-FLIP was detected by immunohistochemieal method in 35 ca-ses of gallbladder cancer compared with 10 cases of normal gallbladder tissue and 10 cases of gallblad-der adenoma. After being interfered by RNAi of c-FLIP, cell viability was measured by CCK-8 meth-od, morphological changes observed through phase-microscopy and apoptosis detected by flow cytome-try. Results The positive expression of e-FLIP in gallbladder cancer tissues was significantly higher than that in normal gallbladder tissues(P<0.05) and gallbladder adenoma tissues(P<0. 05). After being interfered by RNAi of c-FLIP, cell growth of gallbladder cancer cell line SGC-996 was markedly inhibited. Either in phase-microscopy or in flow eytometry, the proportion of apoptosis in samples treated with RNAi of c-FliP for 24 hours was notablely increased as compared with those untreated samples(P<0.05). Conclusion Targeted therapy of RNAi of c-FLIP might be a promising method to treat gallbladder cancer.