中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2009年
8期
757-760
,共4页
冯虎%袁峰%郭开今%周冰%陈向阳%龚维成%汤押庚
馮虎%袁峰%郭開今%週冰%陳嚮暘%龔維成%湯押庚
풍호%원봉%곽개금%주빙%진향양%공유성%탕압경
脊髓损伤%诱发电位%模型,动物
脊髓損傷%誘髮電位%模型,動物
척수손상%유발전위%모형,동물
Spinal cord injury%Evoked potentials%Models,animal
目的 建立脊髓损伤精确分级动物模型.方法 自行设计一种犬的运动-静止压迫型脊髓损伤模型,以大脑皮层诱发电位(CSEP)和不同压迫时间为参数,以T13横突连线为中心,安装大小为0.6 cm×1.0 cm的加压阀,以0.2 mm/min速度压迫脊髓,同时持续性CSEP监测,随压迫深度加深,CSEP波幅不断压低,当波幅下降达基础值的50%时停止下压,继续维持压迫,将20只杂种犬随机分为三组:A组(n=8):脊髓继续受压30 min;B组(n=8):脊髓继续受压180 min;C组(n=4):为对照组,脊髓显露后不损伤.观察电生理学、组织病理学、功能恢复及MRI变化.结果 两组脊髓组织学均有损害、MRI显示两组均有脊髓受压性改变,按照Aiith法计算A、B组的脊髓白质残留面积百分比和MRI脊髓变性空洞区最大横面积百分比,差异均有统计学意义(P<0.05);A组CSEP逐渐恢复达基线的76%,B组CSEP无恢复,C组一直无变化;脊髓受压早期两组均有后肢功能障碍,按照改良的Tarlov测定法和运动能力法评估A、B两组,差异有统计学意义(P<0.05).结论 以CSEP和不同压迫时间为参数,能够建立不同损伤程度的可重复性强的分级脊髓损伤模型.
目的 建立脊髓損傷精確分級動物模型.方法 自行設計一種犬的運動-靜止壓迫型脊髓損傷模型,以大腦皮層誘髮電位(CSEP)和不同壓迫時間為參數,以T13橫突連線為中心,安裝大小為0.6 cm×1.0 cm的加壓閥,以0.2 mm/min速度壓迫脊髓,同時持續性CSEP鑑測,隨壓迫深度加深,CSEP波幅不斷壓低,噹波幅下降達基礎值的50%時停止下壓,繼續維持壓迫,將20隻雜種犬隨機分為三組:A組(n=8):脊髓繼續受壓30 min;B組(n=8):脊髓繼續受壓180 min;C組(n=4):為對照組,脊髓顯露後不損傷.觀察電生理學、組織病理學、功能恢複及MRI變化.結果 兩組脊髓組織學均有損害、MRI顯示兩組均有脊髓受壓性改變,按照Aiith法計算A、B組的脊髓白質殘留麵積百分比和MRI脊髓變性空洞區最大橫麵積百分比,差異均有統計學意義(P<0.05);A組CSEP逐漸恢複達基線的76%,B組CSEP無恢複,C組一直無變化;脊髓受壓早期兩組均有後肢功能障礙,按照改良的Tarlov測定法和運動能力法評估A、B兩組,差異有統計學意義(P<0.05).結論 以CSEP和不同壓迫時間為參數,能夠建立不同損傷程度的可重複性彊的分級脊髓損傷模型.
목적 건립척수손상정학분급동물모형.방법 자행설계일충견적운동-정지압박형척수손상모형,이대뇌피층유발전위(CSEP)화불동압박시간위삼수,이T13횡돌련선위중심,안장대소위0.6 cm×1.0 cm적가압벌,이0.2 mm/min속도압박척수,동시지속성CSEP감측,수압박심도가심,CSEP파폭불단압저,당파폭하강체기출치적50%시정지하압,계속유지압박,장20지잡충견수궤분위삼조:A조(n=8):척수계속수압30 min;B조(n=8):척수계속수압180 min;C조(n=4):위대조조,척수현로후불손상.관찰전생이학、조직병이학、공능회복급MRI변화.결과 량조척수조직학균유손해、MRI현시량조균유척수수압성개변,안조Aiith법계산A、B조적척수백질잔류면적백분비화MRI척수변성공동구최대횡면적백분비,차이균유통계학의의(P<0.05);A조CSEP축점회복체기선적76%,B조CSEP무회복,C조일직무변화;척수수압조기량조균유후지공능장애,안조개량적Tarlov측정법화운동능역법평고A、B량조,차이유통계학의의(P<0.05).결론 이CSEP화불동압박시간위삼수,능구건립불동손상정도적가중복성강적분급척수손상모형.
Objective To establish an animal model for accurately grading different degrees of spinal cord injury. Methods Eight dogs underwent sustained compression on spinal cord for 30 minutes in group A and another 8 for 180 minutes in group B using a self-designed device for weight-loading com-pression on spinal cord. Cortex somatosensory evoked potentials (CSEP) were monitored during all the pro-cedures of making the model. Functional motion recovery was judged throughout all the 28 days using the Combined Behavioral Score (CBS) and modified Tarlov system. The volume of the lesion to the tissue was assessed with magnetic imaging and histological analysis. Results The CSEP amplitude in group A re-covered gradually,but there was no recovery in group B. The volume of lesion to the tissue in group A was significantly smaller than that in group B (P<0.05). The behavior improvement in group A was significantly greater than that in group B (P<0.05). Conclusion An ideal,reliable and duplicable animal model can be established for grading spinal cord injury using the 2 parameters of CSEP and time.
