中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2008年
6期
598-601
,共4页
李丽娜%俞东征%海荣%魏建春%付秀萍%张恩民%张志凯%马凤琴%蔡虹%张建华%徐冬蕾
李麗娜%俞東徵%海榮%魏建春%付秀萍%張恩民%張誌凱%馬鳳琴%蔡虹%張建華%徐鼕蕾
리려나%유동정%해영%위건춘%부수평%장은민%장지개%마봉금%채홍%장건화%서동뢰
耶尔森菌,鼠疫%基因组%DNA可移植因子
耶爾森菌,鼠疫%基因組%DNA可移植因子
야이삼균,서역%기인조%DNA가이식인자
Yersiniapestis%Genome%DNA transposable elements
目的 通过鼠疫耶尔森菌基因组中插入序列IS100位点的特异性研究,对我国鼠疫菌基因组的构成类型和特点进行分析.方法 以鼠疫菌东方变种CO92全长序列为参考,选择5个位置的IS100位点,在IS100基因相邻两外侧设计1对引物,在IS100基冈内设汁1对向外引物使其分别能够与两外侧引物扩增.对来自13个生态型的91株鼠疫菌和1株假结核耶尔森菌进行PCR扩增,产物进行克隆测序分析,同时把各菌株的IS100位点情况标记在我国鼠疫疫源地类型慨图上二进行分析.结果 实验所用91株鼠疫菌在所选定IS100位点出现含有和不含IS100的插入序列,基因片段组成相对CO92序列已发生改变,而且这些IS100基因型的组成在我国鼠疫自然疫源地中呈现区域性分布.结论 IS100基因型别分布与我国鼠疫菌疫源地分型分布基本一致,并直接反映了鼠疫菌基因组某特定位点的基因组成状况及其在进化过程中的高度流动性.
目的 通過鼠疫耶爾森菌基因組中插入序列IS100位點的特異性研究,對我國鼠疫菌基因組的構成類型和特點進行分析.方法 以鼠疫菌東方變種CO92全長序列為參攷,選擇5箇位置的IS100位點,在IS100基因相鄰兩外側設計1對引物,在IS100基岡內設汁1對嚮外引物使其分彆能夠與兩外側引物擴增.對來自13箇生態型的91株鼠疫菌和1株假結覈耶爾森菌進行PCR擴增,產物進行剋隆測序分析,同時把各菌株的IS100位點情況標記在我國鼠疫疫源地類型慨圖上二進行分析.結果 實驗所用91株鼠疫菌在所選定IS100位點齣現含有和不含IS100的插入序列,基因片段組成相對CO92序列已髮生改變,而且這些IS100基因型的組成在我國鼠疫自然疫源地中呈現區域性分佈.結論 IS100基因型彆分佈與我國鼠疫菌疫源地分型分佈基本一緻,併直接反映瞭鼠疫菌基因組某特定位點的基因組成狀況及其在進化過程中的高度流動性.
목적 통과서역야이삼균기인조중삽입서렬IS100위점적특이성연구,대아국서역균기인조적구성류형화특점진행분석.방법 이서역균동방변충CO92전장서렬위삼고,선택5개위치적IS100위점,재IS100기인상린량외측설계1대인물,재IS100기강내설즙1대향외인물사기분별능구여량외측인물확증.대래자13개생태형적91주서역균화1주가결핵야이삼균진행PCR확증,산물진행극륭측서분석,동시파각균주적IS100위점정황표기재아국서역역원지류형개도상이진행분석.결과 실험소용91주서역균재소선정IS100위점출현함유화불함IS100적삽입서렬,기인편단조성상대CO92서렬이발생개변,이차저사IS100기인형적조성재아국서역자연역원지중정현구역성분포.결론 IS100기인형별분포여아국서역균역원지분형분포기본일치,병직접반영료서역균기인조모특정위점적기인조성상황급기재진화과정중적고도류동성.
Objective To study the genotyping distribution of the Yersinia pestis(Y.pestis)strains by characterizing the diversity of the insertion sequence IS100 within the Y.pestis genome.Methods Derived fromthe known sequence of oriental strain CO92,5 pairs of locus-specific primers originating from both sides of the adjacent region of IS100 copies were designed,and two other complementary primers inside the IS100 sequence were designed to correspond with the outer primers.Then,91 Y.pestis strains and l pseudotubebculosis strain were tested by the specific PCR method using the primers described above and the PCR products were conformed by the sequence analysis,then further analysis WaS performed after the IS100 status was marked on the map of the plague focus type of china.Results The 91 Y.pestis strains had different IS100 status in their genome on tested loci.some possessed IS100 insertion,some didn't,and others changed their genome constitution.The IS100 possession on the 5 loci also suggested a distribution of regionality.Conclusion The analysis of some IS100 insertion element loci reveals that the IS100 genotyping distribution is consistent with the plague focus of type of China.And IS100genotyping pattern of the Y.pestis stains well reflects its genome constitution and the high flowability in its natural evolution.
