CAJ | 학술논문

目的探讨褪黑素受体1B(melatonin receptor 1B,MTNR1B)基因rs10830963位点单核苷酸多态性与妊娠期糖尿病(gestational diabetes mellitus,GDM)遗传易感性的关系. 方法选择来自浙江省衢州市妇幼保健院的GDM孕妇87例(GDM组)和同期正常妊娠妇女91例(对照组),应用PCR扩增和DNA测序技术确定所有研究对象MTNR1B基因rs10830963位点等位基因和基因型频率分布,并比较2组之间rs10830963位点等位基因和基因型频率的差异.此外,利用Logistic回归分析判断风险等位基因与空腹血糖和糖化血红蛋白(glycosylated hemoglobin A1c,HbA1c)的关系. 结果 GDM组rs10830963位点G等位基因频率和GG基因型频率均明显高于对照组,G等位基因携带者和GG基因型妇女发生GDM危险性分别是C等位基因和CC基因型的1.53倍(OR=1.53,95％ CI:1.005～2.324,P=0.047)和2.16倍(OR=2.16,95％ CI:1.052～4.434,P=0.034).利用Logistic回归校正年龄、孕前体重指数(body mass index,BMI)和糖尿病家族史等因素之后,GG基因型妇女发生GDM的危险性仍然显著增加(OR=2.07,95％ CI:1.048～4.372,P=0.022).多元线性回归分析显示风险等位基因G与空腹血糖(0.068 mmol/L,P=0.015)水平及HbA1c(0.073％,P=0.028)水平呈显著正相关. 结论 MTNR1B基因rsl0830963位点单核苷酸多态性和GDM遗传易感性有关.rs10830963位点G等位基因为GDM发生的遗传风险因子.
목적 탐토퇴흑소수체1B(melatonin receptor 1B,MTNR1B)기인rs10830963위점단핵감산다태성여임신기당뇨병(gestational diabetes mellitus,GDM)유전역감성적관계. 방법 선택래자절강성구주시부유보건원적GDM잉부87례(GDM조)화동기정상임신부녀91례(대조조),응용PCR확증화DNA측서기술학정소유연구대상MTNR1B기인rs10830963위점등위기인화기인형빈솔분포,병비교2조지간rs10830963위점등위기인화기인형빈솔적차이.차외,이용Logistic회귀분석판단풍험등위기인여공복혈당화당화혈홍단백(glycosylated hemoglobin A1c,HbA1c)적관계. 결과 GDM조rs10830963위점G등위기인빈솔화GG기인형빈솔균명현고우대조조,G등위기인휴대자화GG기인형부녀발생GDM위험성분별시C등위기인화CC기인형적1.53배(OR=1.53,95％ CI:1.005～2.324,P=0.047)화2.16배(OR=2.16,95％ CI:1.052～4.434,P=0.034).이용Logistic회귀교정년령、잉전체중지수(body mass index,BMI)화당뇨병가족사등인소지후,GG기인형부녀발생GDM적위험성잉연현저증가(OR=2.07,95％ CI:1.048～4.372,P=0.022).다원선성회귀분석현시풍험등위기인G여공복혈당(0.068 mmol/L,P=0.015)수평급HbA1c(0.073％,P=0.028)수평정현저정상관. 결론 MTNR1B기인rsl0830963위점단핵감산다태성화GDM유전역감성유관.rs10830963위점G등위기인위GDM발생적유전풍험인자.
Objective To investigate whether the genetic variant rs10830963 of melatonin receptor 1B(MTNR1B)gene is associated with increased risk for gestational diabetes mellitus (GDM).Methods Eighty-seven GDM subjects(GDM group)and 91 normal pregnant women (control group)were randomly recruited form Women and Children's Hospital of Quzhou,Zhejiang Province,China.The allele and genotype frequencies of the rsi0830963 in MTNR1B gene were determined in all participants with PCR amplification and DNA sequencing.The allele and genotype frequencies of rs10830963 were compared to determine their differences between GDM subjects and normal controls.In addition,multiple linear regression was conducted to investigate the association patterns of the risk allele with fasting glucose and HbAlc levels.Results Both GG genotype and G allele frequencies of the rs10830963 loci in the GDM group were significantly higher than those in the controls,and women with G allele and GG genotype were associated with increased GDM risk(OR=1.53,95％ CI:1.005-2.324,P=0.047 and OR=2.16,95％ CI:1.052-4.434,P=0.034 respectively).After adjusting for age,body mass index before pregnancy,and family history of diabetes mellitus,women carrying GG genotype still had a higher GDM risk(OR =2.07,95％ CI:1.048-4.372,P =0.022).Multiple linear regression showed that the rs10830963 G allele was positively correlated with higher levels of fasting glucose(0.068 mmol/L,P=0.015)and HbAlc(0.073％,P=0.028).Conclusions Genetic variant rs10830963 in MTNR1B gene may contribute to the susceptibility to GDM in Chinese population and the rs10830963 G allele is a risk factor for the GDM susceptibility.