CAJ | 학술논문

目的探讨姜黄素的抗心肌纤维化作用对家兔慢性心力衰竭(心衰)的影响.方法 30只新西兰大耳白兔随机分为对照组、心衰组、姜黄素组,每组10只.心衰组和姜黄素组采用主动脉瓣反流及主动脉缩窄法制作心衰模型,对照组做假手术但不造成主动脉瓣反流和主动脉缩窄.姜黄素组口服姜黄素(100 nag·kg-1·d-1),对照组和心衰组口服安慰剂.主动脉瓣反流术及其假手术前,主动脉缩窄术及其假手术后8周,所有动物做2次心脏超声检查.第2次超声检查后,取出心脏行基质金属蛋白酶(MMP)-2和MMP-9的免疫组织化学染色和Masson染色,观察MMP表达的改变和心肌纤维化情况.结果前后2次超声结果比较,心衰组左室射血分数和左室内径缩短分数显著下降,左室内径、左室后壁和室间隔厚度显著增大;姜黄素组上述指标也有显著改变,但程度较心衰组轻;对照组上述指标无显著改变.Masson染色显示,心衰组胶原含量高于姜黄素组和对照组(P<0.05);免疫组织化学染色显示,心衰组MMP-2和MMP-9的表达低于姜黄素组和对照组(P<0.05).结论姜黄素通过提高MMP的表达可减轻心衰时的心肌纤维化,该作用可能是其改善心衰时心肌重构和心脏收缩功能的重要机制之一.抗纤维化治疗有可能成为心衰治疗的新方法 .
목적 탐토강황소적항심기섬유화작용대가토만성심력쇠갈(심쇠)적영향.방법 30지신서란대이백토수궤분위대조조、심쇠조、강황소조,매조10지.심쇠조화강황소조채용주동맥판반류급주동맥축착법제작심쇠모형,대조조주가수술단불조성주동맥판반류화주동맥축착.강황소조구복강황소(100 nag·kg-1·d-1),대조조화심쇠조구복안위제.주동맥판반류술급기가수술전,주동맥축착술급기가수술후8주,소유동물주2차심장초성검사.제2차초성검사후,취출심장행기질금속단백매(MMP)-2화MMP-9적면역조직화학염색화Masson염색,관찰MMP표체적개변화심기섬유화정황.결과 전후2차초성결과 비교,심쇠조좌실사혈분수화좌실내경축단분수현저하강,좌실내경、좌실후벽화실간격후도현저증대;강황소조상술지표야유현저개변,단정도교심쇠조경;대조조상술지표무현저개변.Masson염색현시,심쇠조효원함량고우강황소조화대조조(P<0.05);면역조직화학염색현시,심쇠조MMP-2화MMP-9적표체저우강황소조화대조조(P<0.05).결론 강황소통과제고MMP적표체가감경심쇠시적심기섬유화,해작용가능시기개선심쇠시심기중구화심장수축공능적중요궤제지일.항섬유화치료유가능성위심쇠치료적신방법 .
Objective To investigate the effects of Curcumin on rabbits with chronic heart failure. Methods Heart failure was induced by combined aortic regurgitation and aortic stenosls in 20 New for 8 weeks, 10 sham operated rabbits served as controls (Con). Echocardiography was performed in all rabbits at baseline and 8 weeks later. Aortic diameter (AO), left ventricular ejection fraction (LVEF), left ventricular fractional shortening ( LVFS), left ventricular end-systolic dimension (LVDs), left ventricular end-diastolic dimension ( LVDd), left ventricular posterior wall thickness (LVPW) and interventricular septum thickness (IVS) were measured. Myocardial matrix metalloproteinase (MMP)-2 and MMP-9 expressions and fibrosis were determined by immunohistochemistry and Masaon staining respectively. Results Compared to baseline, LVEF and LVFS were significantly decreased, AO, LVDs, LVDd, LVPW, and IVS significantly increased at 8 weeks after operation in HF group hile these changes could be significantly attenuated in Curcumin treated rabbits. The protein expressions of MMP-2 and MMP-9 were significantly down-regulated in HF group and could be significantly up-regnlated by Curcumin treatment. The increased collagen deposition in HF group was also significantly reduced by Cureumin treatment. Conclusion Curcumin attenuated left ventricular dysfunction and remodeling by up-regulating MMPs expressions and reducing myocardial fibrosis.