中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2009年
5期
304-307
,共4页
许瑾%杨川华%顾伟齐%李绪行%萧树东
許瑾%楊川華%顧偉齊%李緒行%蕭樹東
허근%양천화%고위제%리서행%소수동
溃疡性结肠炎%克罗恩病%表面增强激光解析电离飞行质谱技术
潰瘍性結腸炎%剋囉恩病%錶麵增彊激光解析電離飛行質譜技術
궤양성결장염%극라은병%표면증강격광해석전리비행질보기술
Ulcerative colitis%Crohn's disease%Spectrometry,mass,matrix-assisted laser desorption-ionization
目的 应用表面增强激光解析电离飞行质谱技术(SELDI-TOF-MS)寻找溃疡性结肠炎(UC)和克罗恩病(CD)患者的血浆差异表达蛋白,建立筛选模型.方法 采用CM10芯片对24例UC、25例CD和25例正常对照者血浆进行分析,从蛋白表达图谱中发现差异蛋白,建立区分CD和正常对照、UC和正常对照、UC和CD以及炎症性肠病(IBD)和正常对照的决策树分析模型,并进行盲法筛选.结果 在质荷比(m/z)2000～30 000范围内,CD和正常对照之间、UC和正常对照之间、IBD和正常对照之间具有2倍以上差异的蛋白峰数分别为9个、5个和11个(P＜0.05),软件自动选取m/z为8208、8837的蛋白质建立区分CD和正常对照的决策树模型,m/z为6985的蛋白质建立区分UC和正常对照的决策树模型,m/z为8208、1752、28 840和1702的4个蛋白质建立IBD和正常对照的决策树模型,3个决策树模型的灵敏度分别为96%、82%、91%,特异度分别为100%、85%、100%.结论 m/z 8208对于CD筛选价值高,特异度好,值得进一步探索.
目的 應用錶麵增彊激光解析電離飛行質譜技術(SELDI-TOF-MS)尋找潰瘍性結腸炎(UC)和剋囉恩病(CD)患者的血漿差異錶達蛋白,建立篩選模型.方法 採用CM10芯片對24例UC、25例CD和25例正常對照者血漿進行分析,從蛋白錶達圖譜中髮現差異蛋白,建立區分CD和正常對照、UC和正常對照、UC和CD以及炎癥性腸病(IBD)和正常對照的決策樹分析模型,併進行盲法篩選.結果 在質荷比(m/z)2000～30 000範圍內,CD和正常對照之間、UC和正常對照之間、IBD和正常對照之間具有2倍以上差異的蛋白峰數分彆為9箇、5箇和11箇(P＜0.05),軟件自動選取m/z為8208、8837的蛋白質建立區分CD和正常對照的決策樹模型,m/z為6985的蛋白質建立區分UC和正常對照的決策樹模型,m/z為8208、1752、28 840和1702的4箇蛋白質建立IBD和正常對照的決策樹模型,3箇決策樹模型的靈敏度分彆為96%、82%、91%,特異度分彆為100%、85%、100%.結論 m/z 8208對于CD篩選價值高,特異度好,值得進一步探索.
목적 응용표면증강격광해석전리비행질보기술(SELDI-TOF-MS)심조궤양성결장염(UC)화극라은병(CD)환자적혈장차이표체단백,건립사선모형.방법 채용CM10심편대24례UC、25례CD화25례정상대조자혈장진행분석,종단백표체도보중발현차이단백,건립구분CD화정상대조、UC화정상대조、UC화CD이급염증성장병(IBD)화정상대조적결책수분석모형,병진행맹법사선.결과 재질하비(m/z)2000～30 000범위내,CD화정상대조지간、UC화정상대조지간、IBD화정상대조지간구유2배이상차이적단백봉수분별위9개、5개화11개(P＜0.05),연건자동선취m/z위8208、8837적단백질건립구분CD화정상대조적결책수모형,m/z위6985적단백질건립구분UC화정상대조적결책수모형,m/z위8208、1752、28 840화1702적4개단백질건립IBD화정상대조적결책수모형,3개결책수모형적령민도분별위96%、82%、91%,특이도분별위100%、85%、100%.결론 m/z 8208대우CD사선개치고,특이도호,치득진일보탐색.
Objective To screen the plasma differential expressed proteins in patients with Crohn's disease(CD)and ulcerative colitis(UC)using surface-enhanced laser desorption ionizationtime of flight mass spectrometry,and to establish decision trees algorithms.Methods The plasma samples from 24 UC patients,25 CD patients and 25 healthy controls were analyzed with CM10 protein chip.The proteomic spectra of CD,UC and inflammatory bowel disease(IBD)were compared with controls respectively.The differential proteins that significantly altered expression levels were selected to establish decision trees algorithms of CD.UC and IBD and then blind validations were tested.Results In the range of m/z 2000-30 000,differential expressed proteins that changed at least 2-fold between CD and controls were 9.between UC and controls were 5,and between IBD and controls were 11(P＜0.05).The software automatically picked up the m/z 8208 and 8837 as decision trees algorithms for differentiating CD from controls and m/z 6985 for differentiating UC from controls as well as m/z 8208,1752,28840 and 1702 for differentiating IBD from controls.The sensitivities of decision trees algorithms for CD,UC and IBD were 96%,82%and 91%respectively,and the specificities were 100%,85%and 100%respectively.Conclusions The protein of m/z 8208 which has high sensitivity in differentiating CD from controls is worthy of further study.
