CAJ | 학술논문

目的研究表皮-钙黏附素(E-CD)和Snail蛋白在大肠癌组织中的表达及其与大肠癌侵袭、转移和预后之间的关系.方法采用免疫组化EnVision法,检测E-CD和Snail蛋白在30例正常大肠黏膜、30例大肠腺瘤及142例大肠癌组织中的表达.结果 E-CD蛋白在正常大肠黏膜组织中的强阳性表达率为90.0%,明显高于其在大肠腺瘤和大肠癌组织中的强阳性表达率(63.3%和41.5%,P<0.05).Snail蛋白在大肠癌组织中的阳性表达率为52.1%,明显高于其在正常大肠黏膜和大肠腺瘤组织中的阳性表达率(6.7%和26.7%,P<0.05).E-CD蛋白的表达与大肠癌的分化程度、浸润深度、静脉侵犯、淋巴管侵犯、淋巴结转移以及Duke's分期有关(均P<0.05),而与患者的年龄、性别、肿瘤大小以及病理组织学类型无关(均P0.05).Snail蛋白的表达与大肠癌的分化程度、浸润深度、静脉侵犯、淋巴管侵犯、淋巴结转移、病理组织学类型以及Duke's分期有关(均P<0.05),而与患者的年龄、性别以及肿瘤大小无关(均P>0.05).E-CD蛋白与Snail蛋自在大肠癌组织中的表达呈负相关(r=-0.508).E-CD蛋白阳性表达者的术后1、3和5年生存率明显高于阴性表达者(P<0.05),Snail蛋白阴性表达者的术后1、3和5年生存率明显高于阳性表达者(P<0.05).淋巴结转移、Duke's分期、E-CD和Snail蛋白的表达可作为大肠癌独立的预后指标(均P<0.05).结论 E-CD和Snail蛋白的表达与大肠癌的侵袭、转移和预后明显相关,E-CD蛋白表达降低和Snail蛋白表达增强的大肠癌患者病期晚、预后差.
목적 연구표피-개점부소(E-CD)화Snail단백재대장암조직중적표체급기여대장암침습、전이화예후지간적관계.방법 채용면역조화EnVision법,검측E-CD화Snail단백재30례정상대장점막、30례대장선류급142례대장암조직중적표체.결과 E-CD단백재정상대장점막조직중적강양성표체솔위90.0%,명현고우기재대장선류화대장암조직중적강양성표체솔(63.3%화41.5%,P<0.05).Snail단백재대장암조직중적양성표체솔위52.1%,명현고우기재정상대장점막화대장선류조직중적양성표체솔(6.7%화26.7%,P<0.05).E-CD단백적표체여대장암적분화정도、침윤심도、정맥침범、림파관침범、림파결전이이급Duke's분기유관(균P<0.05),이여환자적년령、성별、종류대소이급병리조직학류형무관(균P0.05).Snail단백적표체여대장암적분화정도、침윤심도、정맥침범、림파관침범、림파결전이、병리조직학류형이급Duke's분기유관(균P<0.05),이여환자적년령、성별이급종류대소무관(균P>0.05).E-CD단백여Snail단자재대장암조직중적표체정부상관(r=-0.508).E-CD단백양성표체자적술후1、3화5년생존솔명현고우음성표체자(P<0.05),Snail단백음성표체자적술후1、3화5년생존솔명현고우양성표체자(P<0.05).림파결전이、Duke's분기、E-CD화Snail단백적표체가작위대장암독립적예후지표(균P<0.05).결론 E-CD화Snail단백적표체여대장암적침습、전이화예후명현상관,E-CD단백표체강저화Snail단백표체증강적대장암환자병기만、예후차.
Objective To study the E-CD and Snail expressions in colorectal cancer and their relationship with colorectal cancer invasion, metastasis and prognosis. Methods Immunohistochemical staining (EnVision) was used to detect the E-CD and Snail expressions in 30 normal colorectal mucosa, 30 colorectal adenoma and 142 colorectal cancer tissues. Results E-CD in the normal colorectal mucosa was strongly positive expressed (90.0%), significantly higher than that in eolorectal adenomas (63.3%) and coloreetal cancer tissues (41.5%). E-CD expression was significantly related to tumor differentiation, invasion depth, vascular invasion, lymph node metastasis and Dukes' stage (P<0.05), but not to the patients' age, gender, tumor size and tumor histological type (P>0.05 ). The 1-, 3- and 5-year survival rates of the E-CD positive patients with colorectal cancer were significantly higher than that in E-CD negative patients. The positive expression rate of Snail in colorectal cancer tissues (52. 1%) was significantly higher than that in normal colorectal mucosa ( 6.7% ) and colorectal adenomas ( 26.7%, P<0.05 ). The snail expression was significantly correlated to tumor histological type, differentiation, invasion depth, vascular invasion, lymph node metastasis and Duke's stage (P<0.05), but not to patients' age, sex and tumor size (P>0.05 ), The 1-, 3- and 5-year survival rates of Snail negative patients with colorectal cancer was significantly higher than that in patients with positive expression (P<0.05). The expressions of E-CD and Snail in colorectal cancer tissues were inversely correlated (P<0.05). Cox multivariate analysis showed that E-CD and Snail can be used as independent prognostic indicators (P < 0.05). Conclusion E-CD and Snail expressions in colorectal cancer are related to the tumor invasion, metastasis and prognosis. Low expression of E-CD and high expression of Snail are related to the advanced stage, and poor prognosis in colorectal cancer patients. E-CD and Snail can be used as independent prognostic indicators.