CAJ | 학술논문

目的研究肥胖对雌性大鼠青春期启动和生殖功能的影响.方法将雌性SD大鼠在出生当日分成2组:(1)实验组(SL组),小窝喂养,每窝4只；(2)对照组(NL组),正常喂养,每窝12只.每周测体重,生后28 d开始监测青春期启动(阴道口初开放)时间和排卵周期的变化.生后6周行卵巢切除术,测量卵泡内膜的厚度变化；用免疫组化方法测定卵巢交感神经兴奋性的改变；用原位杂交方法测定中枢交感神经核团——蓝斑核的TH mRNA的表达变化.结果与NL组相比,SL组青春期启动明显提早[ SL( 34.3±0.3)d比NL(36.3±0.3)d],排卵周期显著减少(SL 35.0％比NL 66.7％),卵泡内膜厚度明显增加[ SL(14.8±0.3) μm比NL(11.3±0.2)μm],卵巢的TH免疫染色明显增强和蓝斑核的TH mRNA表达明显增加,其差异均有统计学意义(均P＜0.05).结论肥胖对雌鼠青春期的启动和生殖功能有明显的影响.
목적 연구비반대자성대서청춘기계동화생식공능적영향.방법 장자성SD대서재출생당일분성2조:(1)실험조(SL조),소와위양,매와4지；(2)대조조(NL조),정상위양,매와12지.매주측체중,생후28 d개시감측청춘기계동(음도구초개방)시간화배란주기적변화.생후6주행란소절제술,측량란포내막적후도변화；용면역조화방법측정란소교감신경흥강성적개변；용원위잡교방법측정중추교감신경핵단——람반핵적TH mRNA적표체변화.결과 여NL조상비,SL조청춘기계동명현제조[ SL( 34.3±0.3)d비NL(36.3±0.3)d],배란주기현저감소(SL 35.0％비NL 66.7％),란포내막후도명현증가[ SL(14.8±0.3) μm비NL(11.3±0.2)μm],란소적TH면역염색명현증강화람반핵적TH mRNA표체명현증가,기차이균유통계학의의(균P＜0.05).결론 비반대자서청춘기적계동화생식공능유명현적영향.
Objective To investigate the effects of obesity on the lipid and glucose metabolism in female rats.Methods The litter size was adjusted to 4 pups per litter for the small litter ( SL,treatment group) and 12 neonates per dam in the normal litter (NL,controls) at postnatal day 0.Body weights were measured weekly.At the age of 6 weeks,bilateral ovariectomy was performed.Tbe blood levels of free fat acid,triglyceride,total cholesterol,free blood glucose and free insulin were evaluated. The muscular expression of AMP-activated protein kinase was detected by immune staining.Results As compared to normal litters,the levels of fat acid,triglyceride,total cholesterol,free blood glucose and free insulin significantly increased.The muscular activity of AMP-activated protein kinase decreased.All difference were statistically significant (P ＜ 0.05).Conclusion Obesity in early life has obvious effects on the lipid and glucose metabolism in female rats.