CAJ | 학술논문

目的研究瑞香素全外抗疟作用与其铁螯合能力的关系.方法在亚性疟原虫FCC1株常规方法体外培养中检测瑞香素和去铁胺杀裂殖体活性.利用荧光探针.利用荧光探针calcein测定瑞香素和去铁胺的铁螯合能力.结果与强铁螯合剂去铁胺相比,瑞香素具有中度的铁螯合能力.体外实验中瑞香素在0～12μmol/L剂量范围内有剂量依赖性的杀裂殖体活性.瑞香素与Fe2+按2:1混合后,抗疟作用明显下降.结论瑞香素的抗疟作用与它的铁螯合能力有关.
목적연구서향소전외항학작용여기철오합능력적관계.방법재아성학원충FCC1주상규방법체외배양중검측서향소화거철알살렬식체활성.이용형광탐침.이용형광탐침calcein측정서향소화거철알적철오합능력.결과여강철오합제거철알상비,서향소구유중도적철오합능력.체외실험중서향소재0～12μmol/L제량범위내유제량의뢰성적살렬식체활성.서향소여Fe2+안2:1혼합후,항학작용명현하강.결론서향소적항학작용여타적철오합능력유관.
Objective To investigate the in vitro antimalarial effect of daphnetin relating to its iron-chelating activity.Methods Schizontocidal activity of daphnetin and desferrioxamine B was tested through an in vitro assay based on the routine in vitro cultivation of Plasmodium faciparum FCC1 strain. The iron-chelating ability of each was measured by the fluorescent probe calcein. Results Daphnetin exhibited a modest iron-chelating ability compared with the powerful ironchelator desferrioxamine B. In vitro test at the range of 0 - 12 μmol/L daphnetin showed a dose-dependent schizontocidal activity which could be inhibited if mixed with Fe2 + in a ratio of 2:1. Conclusion The dose-dependent antimalarial activity of daphnetin is related to its iron-chelating activity.