CAJ | 학술논문

基于蛋白质的加入使功能性CuS纳米粒子的共振光散射明显增强,建立了定量测定蛋白质的新方法.最佳实验条件下,对人血清球蛋白的线性范围为0.1-7.0 μg ml-1,检出限为13 ng ml-1,对人血清白蛋白的线性范围为0.25-11.0 μg ml-1,检出限为26 ng ml-1.该方法成功用于人血清样品的测定,方法简单、灵敏、稳定、选择性好.
기우단백질적가입사공능성CuS납미입자적공진광산사명현증강,건립료정량측정단백질적신방법.최가실험조건하,대인혈청구단백적선성범위위0.1-7.0 μg ml-1,검출한위13 ng ml-1,대인혈청백단백적선성범위위0.25-11.0 μg ml-1,검출한위26 ng ml-1.해방법성공용우인혈청양품적측정,방법간단、령민、은정、선택성호.
Based on the strong enhancement effect of proteins on resonance light scattering of functionalized nano-CuS at 383 nm, a new direct quantitative determination method for proteins in blood serum samples is established. Under optimal conditions, the enhanced RLS intensity is in proportion to the globulin concentration in the range 0.1-7.0 (g ml-1. The limit of detection is 13 ng ml-1. And the dynamic range for human serum albumin is 0.25-11.0 (g ml-1 with detection limit of 26 ng ml-1. Human blood serum samples were determined satisfactorily. This method is characterized by high sensitivity, simplicity, good selectivity and stability of the chemical system.