生物技术通报
生物技術通報
생물기술통보
BIOTECHNOLOGY BULLETIN
2009年
7期
94-97,103
,共5页
大枣%多糖%木瓜蛋白酶%酶解%提取
大棘%多糖%木瓜蛋白酶%酶解%提取
대조%다당%목과단백매%매해%제취
Jujuba Polysaccharide Papain Enzymolysis Extraction
讨论了从提取环磷酸腺苷后的枣汁中提取枣多糖的最佳工艺条件,包括枣汁浓缩4倍,加无水乙醇调枣汁中乙醇体积分数为60%,醇沉5 h;木瓜蛋白酶脱蛋白效果最佳,木瓜蛋白酶液与枣汁的体积比为0.4∶ 1,温度60℃、pH5.0,酶解90 min,蛋白脱除率可达91.8%;AB-8树脂脱色,脱色率为73.64%,多糖得率为94.4%.红外光谱显示,提取的多糖与常规方法提取的多糖成分相同.
討論瞭從提取環燐痠腺苷後的棘汁中提取棘多糖的最佳工藝條件,包括棘汁濃縮4倍,加無水乙醇調棘汁中乙醇體積分數為60%,醇沉5 h;木瓜蛋白酶脫蛋白效果最佳,木瓜蛋白酶液與棘汁的體積比為0.4∶ 1,溫度60℃、pH5.0,酶解90 min,蛋白脫除率可達91.8%;AB-8樹脂脫色,脫色率為73.64%,多糖得率為94.4%.紅外光譜顯示,提取的多糖與常規方法提取的多糖成分相同.
토론료종제취배린산선감후적조즙중제취조다당적최가공예조건,포괄조즙농축4배,가무수을순조조즙중을순체적분수위60%,순침5 h;목과단백매탈단백효과최가,목과단백매액여조즙적체적비위0.4∶ 1,온도60℃、pH5.0,매해90 min,단백탈제솔가체91.8%;AB-8수지탈색,탈색솔위73.64%,다당득솔위94.4%.홍외광보현시,제취적다당여상규방법제취적다당성분상동.
This paper studied technology of extracting polysaccharide from jujube juice which has been extracted c-AMP. The extracting liquid contract 4 times, adding ethanol to make the concentration of alcohol 60%, and time was 5 h. Papain was used to remove protein, and the optimum conditions including 0.4∶ 1V/V papain, temperature 60℃, pH5.0, 90 min. This method may increase the removal rate of protein to 91.8%, and the loss rate of polysaccharides in reduced greatly. The extracted solution was then decolorized through macro-reticular adsorption resin column. The rate was 73.64% and the yield of polysaccharide was 94.4%. The structure was similar to the polysaccharide extracted by normal technology.