热带作物学报
熱帶作物學報
열대작물학보
CHINESE JOURNAL OF TROPICAL CROPS
2009年
11期
1637-1640
,共4页
孙小龙%和丽岗%梁国平%宁连云
孫小龍%和麗崗%樑國平%寧連雲
손소룡%화려강%량국평%저련운
橡胶树%组织培养%正交设计
橡膠樹%組織培養%正交設計
상효수%조직배양%정교설계
rubber tree%tissue culture%orthogonal design.
采用正交设计方法,研究橡胶树品种云研77-2茎段快繁试验中NAA、KT、6-BA 3种激素对茎芽诱导的影响.结果表明,培养基中添加6-BA对茎芽诱导起主导性的作用.橡胶树云研77-2茎芽诱导最适培养基为MS+2.0 mg/L 6-BA+0.1 mg/L NAA+0～0.5 mg/L KT.诱导生根试验中,培养基为1/2 MS+0.5 mg/L IBA的生根率可达66.7%.叶片剪去1/2的自根无性系进行移栽的植株平均成活率为43.6%.
採用正交設計方法,研究橡膠樹品種雲研77-2莖段快繁試驗中NAA、KT、6-BA 3種激素對莖芽誘導的影響.結果錶明,培養基中添加6-BA對莖芽誘導起主導性的作用.橡膠樹雲研77-2莖芽誘導最適培養基為MS+2.0 mg/L 6-BA+0.1 mg/L NAA+0～0.5 mg/L KT.誘導生根試驗中,培養基為1/2 MS+0.5 mg/L IBA的生根率可達66.7%.葉片剪去1/2的自根無性繫進行移栽的植株平均成活率為43.6%.
채용정교설계방법,연구상효수품충운연77-2경단쾌번시험중NAA、KT、6-BA 3충격소대경아유도적영향.결과표명,배양기중첨가6-BA대경아유도기주도성적작용.상효수운연77-2경아유도최괄배양기위MS+2.0 mg/L 6-BA+0.1 mg/L NAA+0～0.5 mg/L KT.유도생근시험중,배양기위1/2 MS+0.5 mg/L IBA적생근솔가체66.7%.협편전거1/2적자근무성계진행이재적식주평균성활솔위43.6%.
Three hormones, NAA, KT and 6-BA, were used to induce stem segments for rapid propagation of clone Yunyan77-2, and an orthogonal design was arranged. The results showed that the medium in the presence of 8-BA had a dominant role in inducing shoots from the stem segments. The optimal shoot inducing medium was MS + 2.0 mg/L 6-BA + 0.1 mg/L NAA + (0～0.5) mg/L KT. The root inducing medium of 1/2MS + 0.5 mg/L IBA produced a rooting rate of 66.7%. The self-rooted plantlets with 1/2 leaves cut had a survival rate of 43.6% after transplanted.
