CAJ | 학술논문

目的探讨曲古抑菌素A(TSA)在体外诱导急性早幼粒白血病细胞HL-60分化的作用.方法采用溴化四甲基偶氮唑蓝法检测TSA对HL-60细胞增殖的影响,流式细胞术检测细胞周期;通过检测细胞分化特异性抗原CD11b的表达研究细胞分化作用;逆转录-聚合酶链反应(RT-PCR)方法检测c-myc基因mRNA的表达.结果经TSA作用后,细胞增殖受抑制,细胞周期阻滞在G0和G1期,P<0.01;TSA作用48 h后,细胞分化率达15.24%;c-myc表达随TSA作用时间延长而降低.结论 TSA对HL-60细胞具有抑制增殖和诱导分化作用.
목적 탐토곡고억균소A(TSA)재체외유도급성조유립백혈병세포HL-60분화적작용.방법 채용추화사갑기우담서람법검측TSA대HL-60세포증식적영향,류식세포술검측세포주기;통과검측세포분화특이성항원CD11b적표체연구세포분화작용;역전록-취합매련반응(RT-PCR)방법검측c-myc기인mRNA적표체.결과 경TSA작용후,세포증식수억제,세포주기조체재G0화G1기,P<0.01;TSA작용48 h후,세포분화솔체15.24%;c-myc표체수TSA작용시간연장이강저.결론 TSA대HL-60세포구유억제증식화유도분화작용.
Objective To detect whether trichostatin(TSA) can induce HL-60 differentiation in vitro. Methods MTT method was used to test the effect of TSA on HL-60 cell growth. Cell cycle was tested by flow cytometry. CD11b expression was tested for detecting cell differentiation, RT-PCR was used to detect the mRNA expression of c-myc in the cells treated by TSA. Results Down-regulation of cell proliferation was observed and cells significantly accumulated at the G0 and G1 phase in HL-60 cells treated with TSA( P <0. 01). Dif-ferentiation rate was 15. 24% after being treated by TSA for 48 h. mRNA of c-myc was down regulated in time-dependent manner. Conclusion TSA can inhibit proliferation and induce differentiation in HL-60 cells.