CAJ | 학술논문

目的研究弓形虫感染早孕期绒毛膜滋养层细胞中人类白细胞抗原G(human leukocyte antigen G,HLA-C)表达水平的变化,探讨弓形虫感染致不良妊娠的分子免疫机制.方法选取正常妊娠6～12周妇女行人工流产术后的绒毛组织,分离滋养层细胞.将绒毛膜滋养层细胞分为实验组和对照组,实验组加入1×104个弓形虫,对照组加入等量生理盐水,培养48 h后,采用实时荧光定量PCR检测各组人绒毛膜滋养层细胞HLA-G mRNA表达水平,流式细胞技术检测各组人绒毛膜滋养层细胞中HLA-G阳性细胞亚群数量.结果实验组和对照组HLA-G mRNA平均表达水平分别为0.15±0.04和0.29±0.07,两组之间的差异具有统计学意义(P＜0.01);实验组绒毛膜滋养层细胞HLA-G阳性细胞亚群的数量为(12.19±2.04)%,对照组为(24.24±3.07)%,两组之间的差异具有统计学意义(P＜0.01).结论早孕期弓形虫感染可下调绒毛膜滋养层细胞HLA-G的表达,减少绒毛膜滋养层细胞中HLA-G阳性细胞亚群数量.
목적 연구궁형충감염조잉기융모막자양층세포중인류백세포항원G(human leukocyte antigen G,HLA-C)표체수평적변화,탐토궁형충감염치불량임신적분자면역궤제.방법 선취정상임신6～12주부녀행인공유산술후적융모조직,분리자양층세포.장융모막자양층세포분위실험조화대조조,실험조가입1×104개궁형충,대조조가입등량생리염수,배양48 h후,채용실시형광정량PCR검측각조인융모막자양층세포HLA-G mRNA표체수평,류식세포기술검측각조인융모막자양층세포중HLA-G양성세포아군수량.결과 실험조화대조조HLA-G mRNA평균표체수평분별위0.15±0.04화0.29±0.07,량조지간적차이구유통계학의의(P＜0.01);실험조융모막자양층세포HLA-G양성세포아군적수량위(12.19±2.04)%,대조조위(24.24±3.07)%,량조지간적차이구유통계학의의(P＜0.01).결론 조잉기궁형충감염가하조융모막자양층세포HLA-G적표체,감소융모막자양층세포중HLA-G양성세포아군수량.
Objective To investigate the effect of infection with Toxoplasma gondii on the human leukocyte antigen G(HLA-G) expression in the human trophoblastic cells and to explore the immune mechanisms in abnormal pregnancy induced by T. gondii. Methods The trophoblastic cells were isolated from the villous tissues, which were obtained from induced abortions of normal pregnancies for 6 to 12 weeks. The trophoblastic cells were divided into experimental and control groups. The experimental group was infected with T. gondii(1 × 104), and the control group was without infection. After being incubated for 48 h, the expression of HLA-G mRNA was analyzed with quantitative real-time PCR. Results Experimental results showed that the average expression levels of HLA-G mRNA in trophoblastic cells were 0. 15 +0.04 in the experimental group,while those in the control group were 0. 29 ± 0. 07. On protein level, the average ratio of HLA-G positive subgroup in the experimental and control group were (12.19 ± 2.04) % and (24.24 ± 3.07) %, respectively.The differences in these groups were statistically significant (P ＜ 0.01). Conclusion From the above observations, it is evident that during the early stage of pregnancy, the expression of HLA-G in the trophoblastic cells was decreased in both mRNA and protein levels due to the infection of T. gondii.