CAJ | 학술논문

目的检测cripto基因mRNA在结直肠癌及其癌旁组织中mRNA表达情况,并探讨其在结直肠癌发生发展中的意义.方法提取36例人大肠癌及癌旁正常组织中总mRNA,采用实时荧光定量PCR法检测其中cripto mRNA表达情况;并分析其表达与患者淋巴结转移、肿瘤分化程度和Dukes's分期相关性.结果 cripto mRNA表达在大肠癌组织中阳性表达率为86.1%(31/36),而正常肠组织未见cripto mRNA表达,统计学差异显著(P<0.0001).cripto mRNA表达与浆膜侵袭、淋巴结转移和Duke's分期密切相关(P<0.05,P<0.05,P>0.05),而与患者肿瘤发生部位和分化程度无关(P>0.05,P>0.05).结论 cripto基因在结直肠癌组织中高表达,可能与大肠侵袭癌转移有关.
목적 검측cripto기인mRNA재결직장암급기암방조직중mRNA표체정황,병탐토기재결직장암발생발전중적의의.방법 제취36례인대장암급암방정상조직중총mRNA,채용실시형광정량PCR법검측기중cripto mRNA표체정황;병분석기표체여환자림파결전이、종류분화정도화Dukes's분기상관성.결과 cripto mRNA표체재대장암조직중양성표체솔위86.1%(31/36),이정상장조직미견cripto mRNA표체,통계학차이현저(P<0.0001).cripto mRNA표체여장막침습、림파결전이화Duke's분기밀절상관(P<0.05,P<0.05,P>0.05),이여환자종류발생부위화분화정도무관(P>0.05,P>0.05).결론 cripto기인재결직장암조직중고표체,가능여대장침습암전이유관.
Objective To investigate the expression and significance of eripto in human eoiorectal car- cinoma. Methods The expression of eripto mRNA in 36 samples of colon cancer tissues and adjacent normal tissues were determined by real time RT-PCR assay,and 2-△△Ct≥2 as a positive standard. Results The positive rate of cripto expression in coiorectal carcinoma tissue is 86. 1% , however, tumor adjacent tissue is 0. Cripto mR- NA was related with scrosa invasion,lymph node metastasis and Duke's stage,but not with tumor location,and pathological grading. Conclusion Cripto is closely correlated to colorectal carcinoma progression, and cripto might be a potential marker for metastasis of colon cancer progression.