中华精神科杂志
中華精神科雜誌
중화정신과잡지
CHINESE JOURNA OF PSYCHIATRY
2012年
3期
161-164
,共4页
刘亮%程灶火%刘晓伟%袁国桢%张会芳%范洁%奚娟%邹凯%陈新宇
劉亮%程竈火%劉曉偉%袁國楨%張會芳%範潔%奚娟%鄒凱%陳新宇
류량%정조화%류효위%원국정%장회방%범길%해연%추개%진신우
精神分裂症%多巴胺质膜转运蛋白质类%受体,多巴胺D2%基因表达%阳性和阴性症状量表
精神分裂癥%多巴胺質膜轉運蛋白質類%受體,多巴胺D2%基因錶達%暘性和陰性癥狀量錶
정신분렬증%다파알질막전운단백질류%수체,다파알D2%기인표체%양성화음성증상량표
Schizophrenia%Dopamine plasma membrane transport proteins%Receptors,dopamine D2%Gene expression%Positive and Negative Syndrome Scale
目的 探讨精神分裂症患者外周血淋巴细胞多巴胺D2受体(DRD2)和多巴胺转运体(DAT)的mRNA表达水平与精神分裂症临床症状的关系.方法 以实时荧光定量逆转录-聚合酶链反应技术检测治疗前精神分裂症组(25例)、长期服药(氯氮平)慢性精神分裂症组(27例)和对照组(30名)外周血淋巴细胞中DRD2和DAT的mRNA表达水平,同时对精神分裂症患者的阳性和阴性症状量表(PANSS)进行评定,并采用Spearman分析二者的相关性.结果 治疗前精神分裂症组、长期服药慢性精神分裂症组、对照组样本外周血淋巴细胞DRD2的基因表达水平分别为0.32±0.13、0.37±0.19、0.34±0.09,3组比较差异无统计学意义(F=0.510,P＞0.05)；DAT的基因表达水平分别为0.48±0.24、0.58±0.21、0.39±0.24,3组比较差异有统计学意义(F=4.330,P＜0.05)；长期服药慢性精神分裂症组DAT基因表达水平显著高于对照组(MS组内=0.194,P ＜0.01)；治疗前精神分裂症组DRD2基因表达水平与PANSS阳性症状分呈显著正相关(r=0.443,P＜0.05),DAT基因表达水平与PANSS总分(r=-0.418,P=0.075)、一般病理症状分(r=-0.434,P=0.063)的相关性未达统计学意义.结论 精神分裂症患者外周血淋巴细胞DRD2的基因表达水平与PANSS量表的阳性症状分显著正相关,而精神分裂症患者外周血淋巴细胞DAT的基因表达水平可能受氯氮平影响上调.
目的 探討精神分裂癥患者外週血淋巴細胞多巴胺D2受體(DRD2)和多巴胺轉運體(DAT)的mRNA錶達水平與精神分裂癥臨床癥狀的關繫.方法 以實時熒光定量逆轉錄-聚閤酶鏈反應技術檢測治療前精神分裂癥組(25例)、長期服藥(氯氮平)慢性精神分裂癥組(27例)和對照組(30名)外週血淋巴細胞中DRD2和DAT的mRNA錶達水平,同時對精神分裂癥患者的暘性和陰性癥狀量錶(PANSS)進行評定,併採用Spearman分析二者的相關性.結果 治療前精神分裂癥組、長期服藥慢性精神分裂癥組、對照組樣本外週血淋巴細胞DRD2的基因錶達水平分彆為0.32±0.13、0.37±0.19、0.34±0.09,3組比較差異無統計學意義(F=0.510,P＞0.05)；DAT的基因錶達水平分彆為0.48±0.24、0.58±0.21、0.39±0.24,3組比較差異有統計學意義(F=4.330,P＜0.05)；長期服藥慢性精神分裂癥組DAT基因錶達水平顯著高于對照組(MS組內=0.194,P ＜0.01)；治療前精神分裂癥組DRD2基因錶達水平與PANSS暘性癥狀分呈顯著正相關(r=0.443,P＜0.05),DAT基因錶達水平與PANSS總分(r=-0.418,P=0.075)、一般病理癥狀分(r=-0.434,P=0.063)的相關性未達統計學意義.結論 精神分裂癥患者外週血淋巴細胞DRD2的基因錶達水平與PANSS量錶的暘性癥狀分顯著正相關,而精神分裂癥患者外週血淋巴細胞DAT的基因錶達水平可能受氯氮平影響上調.
목적 탐토정신분렬증환자외주혈림파세포다파알D2수체(DRD2)화다파알전운체(DAT)적mRNA표체수평여정신분렬증림상증상적관계.방법 이실시형광정량역전록-취합매련반응기술검측치료전정신분렬증조(25례)、장기복약(록담평)만성정신분렬증조(27례)화대조조(30명)외주혈림파세포중DRD2화DAT적mRNA표체수평,동시대정신분렬증환자적양성화음성증상량표(PANSS)진행평정,병채용Spearman분석이자적상관성.결과 치료전정신분렬증조、장기복약만성정신분렬증조、대조조양본외주혈림파세포DRD2적기인표체수평분별위0.32±0.13、0.37±0.19、0.34±0.09,3조비교차이무통계학의의(F=0.510,P＞0.05)；DAT적기인표체수평분별위0.48±0.24、0.58±0.21、0.39±0.24,3조비교차이유통계학의의(F=4.330,P＜0.05)；장기복약만성정신분렬증조DAT기인표체수평현저고우대조조(MS조내=0.194,P ＜0.01)；치료전정신분렬증조DRD2기인표체수평여PANSS양성증상분정현저정상관(r=0.443,P＜0.05),DAT기인표체수평여PANSS총분(r=-0.418,P=0.075)、일반병리증상분(r=-0.434,P=0.063)적상관성미체통계학의의.결론 정신분렬증환자외주혈림파세포DRD2적기인표체수평여PANSS량표적양성증상분현저정상관,이정신분렬증환자외주혈림파세포DAT적기인표체수평가능수록담평영향상조.
Objective Detecting the mRNA expression levels of dopamine D2 receptor (DRD2),dopamine transporter (DAT) genes in the peripheral blood lymphocytes of schizophrenic patients,to explore the relationship between it and the clinic symptoms of schizophrenia Methods Twenty-five cases of schizophrenia patients without medication for at least 1 month,27 chronic schizophrenia patients with longterm clozapine medication,30 normal controls were involved in the study.The mRNA expression of all the subjects was measured by real-time quantitative RT-PCR,and the patients were evaluated with the Positive and Negative Syndrome Scale ( PANSS ).Results The mRNA expression levels of DRD2 gene in schizophrenia patients without medication,chronic patients with clozapine medication and normal controls were ( 0.32 ± 0.13 ),( 0.37 ± 0.19 ),and ( 0.34 ± 0.09 ) respectively,and the difference was not significantly,but the mRNA expression leveis of DAT gene in the three groups were (0.48 ±0.24),(0.58 ±0.21 ) and (0.39 ± 0.24 ) respectively ( F =4.330,P ＜ 0.05 ),and post hoc analysis showed that the mRNA expression levels of DAT in chronic schizophrenia patients were significantly higher than that in normal controls ( P ＜ 0.01 ).The correlation between the mRNA expression levels of DRD2 gene and the scores of positive symptom of PANSS in schizophrenia patients without medication was significant ( r =0.443,P ＜ 0.05 ).Conclusion The results indicate that the mRNA expression levels of DRD2 gene in the peripheral blood lymphocytes are correlated with the positive symptom in schizophrenia patients,and the mRNA expression levels of DAT gene might be over expressed by clozapine medication.
