CAJ | 학술논문

目的探讨超声造影剂介导下重组腺相关病毒载体(rAAV2-GFP)心肌靶向转染前后大鼠左室心肌功能的变化.方法健康成年SD大鼠20只,随机分为对照组与实验组:对照组尾静脉注射超声造影剂+超声照射;实验组注射携带rAAV2-GFP造影剂+超声照射.分别于实验前和实验后14 d利用斑点追踪技术测量大鼠周向应变、收缩期应变率,舒张期应变率、扭转角度,并同时测量大鼠射血分数、短轴缩短率、舒张末期容积及收缩末期容积等左心功能指标.行心肌组织冰冻切片,荧光显微镜下观察GFP的表达情况.结果超声介导重组腺相关病毒心肌靶向转染前后大鼠左室心肌功能指标及相对于心底水平的扭转角度及达峰时间的差异均无统计学意义(P>0.05).结论超声及超声造影剂介导重组腺相关病毒载体靶向转染心肌组织对大鼠左室心肌功能无影响.
목적 탐토초성조영제개도하중조선상관병독재체(rAAV2-GFP)심기파향전염전후대서좌실심기공능적변화.방법 건강성년SD대서20지,수궤분위대조조여실험조:대조조미정맥주사초성조영제+초성조사;실험조주사휴대rAAV2-GFP조영제+초성조사.분별우실험전화실험후14 d이용반점추종기술측량대서주향응변、수축기응변솔,서장기응변솔、뉴전각도,병동시측량대서사혈분수、단축축단솔、서장말기용적급수축말기용적등좌심공능지표.행심기조직빙동절편,형광현미경하관찰GFP적표체정황.결과 초성개도중조선상관병독심기파향전염전후대서좌실심기공능지표급상대우심저수평적뉴전각도급체봉시간적차이균무통계학의의(P>0.05).결론 초성급초성조영제개도중조선상관병독재체파향전염심기조직대대서좌실심기공능무영향.
Objective To study the changes of left ventricular(LV) function before and after rAAV2-GFP targeted to myocardium by the ultrasound mediated microbubbles. Methods Twenty rats were divided into 2 groups. Ten rats underwent ultrasound mediated microbubbles destruction without rAAV2-GFP as control. Another ten rats underwent ultrasound mediated microbubbles destruction containing rAAV2-GFP as experimental group. The mean value of LV rotation of rats was obtained in apical and base plane using speckle tracking imaging(STI) software. LV torsion(LVtor) was defined as apical rotation relative to the base. LV function including circumferential strain,circumferential strain rate of systolic and early diastolic and late diastolic, torsion, time to peak, ejection fraction, fractional shortening, end-diastolic volume, end-systolic volume of LV,heart rate and so on were also measured before and after 14 days. Other rats were killed after 14 days and hearts were harvested. GFP protein expression which showed rAAV2 transfer was observed under fluorescence microscope in frozen section. Results There was no significant difference of LV function of rats before and after rAAV2-GFP myocardium transfection by the ultrasound mediated microhubbles. Conclusions LV myocardial function has no significant difference before and after delivering rAAV2 to the heart in vivo by ultrasound-mediated microbubbles destruction.