中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2012年
6期
451-453
,共3页
高学文%邹艳慧%王萍%云雅丽
高學文%鄒豔慧%王萍%雲雅麗
고학문%추염혜%왕평%운아려
脂肪酸结合蛋白质类%多态性,单核苷酸%脂代谢障碍
脂肪痠結閤蛋白質類%多態性,單覈苷痠%脂代謝障礙
지방산결합단백질류%다태성,단핵감산%지대사장애
Fatty acid-binding proteins%Polymorphism,single nucleotide%Lipid metabolism disorders
目的 调查老年人小肠脂肪酸结合蛋白(I-FABP)基因外显子2中54位点密码子A/T 单核苷酸多态性和不同基因型人群的血脂水平,探讨I-FABP基因多态性与老年人血脂水平的关系.方法 采用聚合酶链反应(PCR)、DNA限制性内切酶酶切等技术对72例汉族老年人54A/T I-FABP基因型进行分析；用全自动生化仪检测入选人群的血浆总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)水平.结果 基因型分组Thr54(-)组、Thr54(+)组各36例,Thr54(-)组与Thr54(+)组比较,TC为(4.50±0.73) mmol/L与(5.48±0.49)mmol/L、TG为(1.08±0.48)mmol/L与(2.02±0.53) mmol/L、LDL-C为(3.10±0.44)mmol/L与(3.50±0.66) mmol/L和HDL-C为(1.14±0.25)mmol/L与(0.96±0.23) mmol/L,差异有统计学意义(t值分别为-6.67、-7.84、-3.03、3.05,均P＜0.05).结论 I-FAB外显子2中54位点密码子A/T SNP与老年人群的血脂水平相关.
目的 調查老年人小腸脂肪痠結閤蛋白(I-FABP)基因外顯子2中54位點密碼子A/T 單覈苷痠多態性和不同基因型人群的血脂水平,探討I-FABP基因多態性與老年人血脂水平的關繫.方法 採用聚閤酶鏈反應(PCR)、DNA限製性內切酶酶切等技術對72例漢族老年人54A/T I-FABP基因型進行分析；用全自動生化儀檢測入選人群的血漿總膽固醇(TC)、三酰甘油(TG)、高密度脂蛋白膽固醇(HDL-C)和低密度脂蛋白膽固醇(LDL-C)水平.結果 基因型分組Thr54(-)組、Thr54(+)組各36例,Thr54(-)組與Thr54(+)組比較,TC為(4.50±0.73) mmol/L與(5.48±0.49)mmol/L、TG為(1.08±0.48)mmol/L與(2.02±0.53) mmol/L、LDL-C為(3.10±0.44)mmol/L與(3.50±0.66) mmol/L和HDL-C為(1.14±0.25)mmol/L與(0.96±0.23) mmol/L,差異有統計學意義(t值分彆為-6.67、-7.84、-3.03、3.05,均P＜0.05).結論 I-FAB外顯子2中54位點密碼子A/T SNP與老年人群的血脂水平相關.
목적 조사노년인소장지방산결합단백(I-FABP)기인외현자2중54위점밀마자A/T 단핵감산다태성화불동기인형인군적혈지수평,탐토I-FABP기인다태성여노년인혈지수평적관계.방법 채용취합매련반응(PCR)、DNA한제성내절매매절등기술대72례한족노년인54A/T I-FABP기인형진행분석；용전자동생화의검측입선인군적혈장총담고순(TC)、삼선감유(TG)、고밀도지단백담고순(HDL-C)화저밀도지단백담고순(LDL-C)수평.결과 기인형분조Thr54(-)조、Thr54(+)조각36례,Thr54(-)조여Thr54(+)조비교,TC위(4.50±0.73) mmol/L여(5.48±0.49)mmol/L、TG위(1.08±0.48)mmol/L여(2.02±0.53) mmol/L、LDL-C위(3.10±0.44)mmol/L여(3.50±0.66) mmol/L화HDL-C위(1.14±0.25)mmol/L여(0.96±0.23) mmol/L,차이유통계학의의(t치분별위-6.67、-7.84、-3.03、3.05,균P＜0.05).결론 I-FAB외현자2중54위점밀마자A/T SNP여노년인군적혈지수평상관.
Objective To investigate relationship between Ala 54 Thr polymorphism in the intestinal fatty acid-binding protein (I-FABP) and plasma lipids in old people.Methods We collected fasting blood samples of selected groups,then we extracted DNA from whole blood,and detected lipids from serum.The 54A/T I-FABP genotypes were analyzed in 72 Han Chinese elderly by polymerase chain reaction (PCR) and DNA restriction enzyming.Total cholesterol (TC),triglyceride (TG),high density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C) were detected.Genotype subgroups were Thr54 (-) and Thr54 (+) groups (36 cases for each).Results Compared with Thr54 (-) genotype group,Thr54 (+) genotype group had higher levels of plasma TC[(4.50±0.73) mmol/L vs.(5.48±0.49)mmol/L],TG[(1.08±0.48) mmol/L vs.(2.02±0.53)mmol/L],LDL-C [(3.10±0.44) mmol/L vs.(3.50±0.66)mmol/L],and had lower level of HDL-C [(1.14±0.25) mmol/L vs.(0.96±0.23)mmol/L](t=-6.67,-7.84,-3.03,3.05,all P＜0.05).
