CAJ | 학술논문

目的:观察高胆固醇血症对大鼠心室肌细胞离子电流的作用.方法:通过全细胞膜片钳技术记录用酶解法分离的正常和高胆固醇饮食的大鼠心室肌细胞离子电流.结果:高胆固醇组(组Ⅱ)血清总胆固醇水平明显高于正常组(组Ⅰ)[(3.10±0.62)mmol·L-1vs(1.18±0.37)mmol·L-1,P＜0.01,n=20].组Ⅱ血清甘油三酯也明显高于组Ⅰ[(1.51±0.30)mmol·L-1vs(0.43±0.15)mmol·L-1,P＜0.01,n=20].组Ⅱ大鼠心室肌细胞动作电位时程(APD)与组Ⅰ相比明显延长,APD50从(70.86±8.12)ms延长至(116.16±6.90)ms(n=10,P＜0.01);APD90从(95.10±7.27)ms延长至(144.04±7.39)ms(n=10,P＜0.01);在实验电压-120 mV,Ik1从(-16.98±4.54)pA/pF(组Ⅰ)增加到(-19.92±4.08)pA/pF(组Ⅱ)(n=12,P＜0.05);在实验电压0 mV,Ica-L从(-8.56±1.29)pA/pF(组Ⅰ)减少到(-5.24±0.90)pA/pF(组Ⅱ)(n=10,P＜0.01);在实验电压+60 mV,Ito从(13.20±1.97)pA/pF(组Ⅰ)减少到(10.30±1.97)pA/pF(组Ⅱ)(n=8,P＜0.05).结论:高胆固醇血症可显著改变心肌细胞离子电流密度的大小,对心脏具有毒性作用.
목적:관찰고담고순혈증대대서심실기세포리자전류적작용.방법:통과전세포막편겸기술기록용매해법분리적정상화고담고순음식적대서심실기세포리자전류.결과:고담고순조(조Ⅱ)혈청총담고순수평명현고우정상조(조Ⅰ)[(3.10±0.62)mmol·L-1vs(1.18±0.37)mmol·L-1,P＜0.01,n=20].조Ⅱ혈청감유삼지야명현고우조Ⅰ[(1.51±0.30)mmol·L-1vs(0.43±0.15)mmol·L-1,P＜0.01,n=20].조Ⅱ대서심실기세포동작전위시정(APD)여조Ⅰ상비명현연장,APD50종(70.86±8.12)ms연장지(116.16±6.90)ms(n=10,P＜0.01);APD90종(95.10±7.27)ms연장지(144.04±7.39)ms(n=10,P＜0.01);재실험전압-120 mV,Ik1종(-16.98±4.54)pA/pF(조Ⅰ)증가도(-19.92±4.08)pA/pF(조Ⅱ)(n=12,P＜0.05);재실험전압0 mV,Ica-L종(-8.56±1.29)pA/pF(조Ⅰ)감소도(-5.24±0.90)pA/pF(조Ⅱ)(n=10,P＜0.01);재실험전압+60 mV,Ito종(13.20±1.97)pA/pF(조Ⅰ)감소도(10.30±1.97)pA/pF(조Ⅱ)(n=8,P＜0.05).결론:고담고순혈증가현저개변심기세포리자전류밀도적대소,대심장구유독성작용.
AIM: To determine whether chronic hypercholesterolemia affects ionic currents on cardiac ventricular myocytes of rats. METHODS: Whole - cell patch - clamp technique was used to record the ionic currents in single cardiac myocytes isolated from normal cholesterolemia and hypercholesterolemia rats. RESULTS: In the hypercholesterol group (group Ⅱ ), serum total - cholesterol level was significantly higher than that of normal group (group Ⅰ) [ (3. 10 ±tricular myocytes of rats, 50% repolarization of action potential duration (APD50) prolonged from (70. 86 ± 8.12) ms (group Ⅰ) to (116.16±6.90)ms (group Ⅱ) (n=10 in each group, P＜0.01); APD90 prolonged from (95.10±7. 27)ms (group Ⅰ ) to (144. 04 ± 7.39)ms (group Ⅱ ) (n = 10 in each group, P ＜ 0. 01 ); at the test potential of - 120 mV, Ik1 increased from ( - 16. 98 ±4. 54) pA/pF(group Ⅰ ) to ( - 19.92 ±4.08) pA/pF (group Ⅱ ) (n = 12 in each group, P ＜ 0. 05 ); at the test potential of 0 mV, ICa- L decreased from ( - 8.56 ± 1.29) pA/pF ( group Ⅰ ) to ( -5. 24 ± 0. 90) pA/pF ( group Ⅱ ) ( n = 10 in each group, P ＜ 0. 01 ); at the test potential of + 60 mV, Ito decreased from (13.20±1.97) pA/pF (group Ⅰ) to (10.30±1.97) pA/pF (group Ⅱ) (n=8 in each group, P＜0. 05). CON-CLUSION: Hypercholesterolemia affects the ionic currents on cardiomyocytes of rats greatly, which may be the ionic mechanism of cardiac toxicity induced by hypercholesterolemia.