国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2008年
12期
1063-1065
,共3页
胃肿瘤%T淋巴细胞%细胞毒性%抗原%CD28%抗原%CD3%抗原%CD4
胃腫瘤%T淋巴細胞%細胞毒性%抗原%CD28%抗原%CD3%抗原%CD4
위종류%T림파세포%세포독성%항원%CD28%항원%CD3%항원%CD4
Stomach eoplasms%T Lymphocytes,Cytotoxic%Antigens,CD28%Antigens,CD3%Antigens,CD4
目的 研究胃癌患者外周血共刺激分子CD28和细胞毒性T淋巴细胞相关抗原-4(CTLA-4-,CD152)及B7分子的表达水平,以探讨胃癌患者共刺激通路是否异常以及在胃癌发病机制中的作用.方法 用流式细胞仪检测56例胃癌患者外周血淋巴细胞表面的CD28、CD152、CD3、CD4、CD8及.B7分子的表达,并与健康对照组及胃良性肿瘤组作比较.结果 胃癌患者外周血中CD3+、CD4+、CD3+ CD28+、B7-1(CD80)、B7-2(CD86)的表达水平及CD4+/CD8+低于健康对照组及良性肿瘤组,CD3+、CDl52+和CD8+水平显著增高.结论 胃癌患者外周血淋巴细胞低表达CD28和B7分子,高表达CTLA-4分子,导致了胃癌患者B7:CDZ8/CTLA-4共刺激通路异常,影响了T细胞彻底清除肿瘤细胞,从而使肿瘤逃避机体的免疫监视、免疫抑制,发生转移.
目的 研究胃癌患者外週血共刺激分子CD28和細胞毒性T淋巴細胞相關抗原-4(CTLA-4-,CD152)及B7分子的錶達水平,以探討胃癌患者共刺激通路是否異常以及在胃癌髮病機製中的作用.方法 用流式細胞儀檢測56例胃癌患者外週血淋巴細胞錶麵的CD28、CD152、CD3、CD4、CD8及.B7分子的錶達,併與健康對照組及胃良性腫瘤組作比較.結果 胃癌患者外週血中CD3+、CD4+、CD3+ CD28+、B7-1(CD80)、B7-2(CD86)的錶達水平及CD4+/CD8+低于健康對照組及良性腫瘤組,CD3+、CDl52+和CD8+水平顯著增高.結論 胃癌患者外週血淋巴細胞低錶達CD28和B7分子,高錶達CTLA-4分子,導緻瞭胃癌患者B7:CDZ8/CTLA-4共刺激通路異常,影響瞭T細胞徹底清除腫瘤細胞,從而使腫瘤逃避機體的免疫鑑視、免疫抑製,髮生轉移.
목적 연구위암환자외주혈공자격분자CD28화세포독성T림파세포상관항원-4(CTLA-4-,CD152)급B7분자적표체수평,이탐토위암환자공자격통로시부이상이급재위암발병궤제중적작용.방법 용류식세포의검측56례위암환자외주혈림파세포표면적CD28、CD152、CD3、CD4、CD8급.B7분자적표체,병여건강대조조급위량성종류조작비교.결과 위암환자외주혈중CD3+、CD4+、CD3+ CD28+、B7-1(CD80)、B7-2(CD86)적표체수평급CD4+/CD8+저우건강대조조급량성종류조,CD3+、CDl52+화CD8+수평현저증고.결론 위암환자외주혈림파세포저표체CD28화B7분자,고표체CTLA-4분자,도치료위암환자B7:CDZ8/CTLA-4공자격통로이상,영향료T세포철저청제종류세포,종이사종류도피궤체적면역감시、면역억제,발생전이.
Objective To investigate.the co-stimulatory pathway of gastric cancer patients by examining the expression of co-stimulatory molecules CD28,CTLA.4(CDl 52)and B7 molecules on peripheral blood lymphocytes(PBLC)in patients with gastric cancer,and to discuss the role of costimulatory pathway in the pathogenesis of gastric cancer.Methods The expression of CD28,CD152,CD3,CD4,CD8 and B7 molecules on peripheral blood lymphocytes and T cell subpopulation were measured by flow cytometry in 56 patients with gastric cancer,and the data was compared and analyzed with that in gastric benign tumor group and healthy control group.Results The expression levels of CD3+,CD4+,CD3+ CD28+,B7-1(CD80),B7-2(CD86)and CD41/CD8+on PBLC in patients with gastric cancer was significantly lower than that of benign stomach tumor group and healthy control group.The expression levels of CD3+ CDl52+ and CD8+ on PBLC in patients with gastric cancer were significantly higher than those of gastric benign tumor group and healthy control group.Conclusion Peripheral lymphocytcs in gastric cancer patients express low level of CD28 and B7 molecules and high level of CDI 52 molecules,which results in dysfunction of B7:CD28/CTLA-4 co-stimulation pathway and in turn affects the capacity of T cell to eliminate tumor cells,which makes tumor cells evading the immune surveillance and metastasizing.
