复旦学报（医学版）
複旦學報（醫學版）
복단학보（의학판）
FUDAN UNIVERSITY JOURNAL OF MEDICAL SCIENCES
2009年
4期
431-434
,共4页
陈坚%钟良%邱冬妮%孙大裕
陳堅%鐘良%邱鼕妮%孫大裕
진견%종량%구동니%손대유
胰腺癌%转化生长因子β1%受体
胰腺癌%轉化生長因子β1%受體
이선암%전화생장인자β1%수체
pancreatic carcinoma%transforming growth factor beta%receptor
目的 分别对具有原发癌特性的BxPC-3及转移癌特性的AsPC-1胰腺癌细胞株中TGFβ1及其Ⅰ型受体TGFβR1 mRNA及蛋白的表达情况进行研究.方法 采用实时荧光定量RT-PCR及Western blot方法检测AsPC-1和BxPC-3胰腺癌细胞中TGFβ1和TGFβR1 mRNA及蛋白的表达.结果 BxPC-3中TGFβ1和TGFβR1的mRNA和蛋白均呈低表达,AsPC-1中TGFβ1和TGFβR1的mRNA和蛋白呈高表达,两者相比较有显著统计学差异(P<0.05).结论 TGFβ1和TGFβR1的高表达可能与胰腺癌的远处转移密切相关.
目的 分彆對具有原髮癌特性的BxPC-3及轉移癌特性的AsPC-1胰腺癌細胞株中TGFβ1及其Ⅰ型受體TGFβR1 mRNA及蛋白的錶達情況進行研究.方法 採用實時熒光定量RT-PCR及Western blot方法檢測AsPC-1和BxPC-3胰腺癌細胞中TGFβ1和TGFβR1 mRNA及蛋白的錶達.結果 BxPC-3中TGFβ1和TGFβR1的mRNA和蛋白均呈低錶達,AsPC-1中TGFβ1和TGFβR1的mRNA和蛋白呈高錶達,兩者相比較有顯著統計學差異(P<0.05).結論 TGFβ1和TGFβR1的高錶達可能與胰腺癌的遠處轉移密切相關.
목적 분별대구유원발암특성적BxPC-3급전이암특성적AsPC-1이선암세포주중TGFβ1급기Ⅰ형수체TGFβR1 mRNA급단백적표체정황진행연구.방법 채용실시형광정량RT-PCR급Western blot방법검측AsPC-1화BxPC-3이선암세포중TGFβ1화TGFβR1 mRNA급단백적표체.결과 BxPC-3중TGFβ1화TGFβR1적mRNA화단백균정저표체,AsPC-1중TGFβ1화TGFβR1적mRNA화단백정고표체,량자상비교유현저통계학차이(P<0.05).결론 TGFβ1화TGFβR1적고표체가능여이선암적원처전이밀절상관.
Objective To compare the different expressions of transforming growth factor beta1 (TGFβ1) and its type one receptor(TGFβR1) between primary pancreatic cancer cell line AsPC-1 and metastatic pancreatic cancer cell line BxPC-3. Methods The mRNA expressions of TGFβ1 and TGFβR1 in AsPC-1 and BxPC-3 pancreatic carcer cell lines were quantatitived by real-time RT-PCR. The protein levels of TGFβ1 and TGFβR1 in these two cell lines were measured by Western blot assay. Results Compared with the primary pancreatic cancer cell AsPC-1, the mRNA and protein expressions of TGFβ1 and TGFβR1 were much higher in BxPC-3 pancreatic cancer cells(P<0.05). Conclusions Upregulations of TGFβ1 and TGFβR1 might be a pivotal incidence in the procedure of malignant progressing and metastasis in pancreatic cancer cells.
