CAJ | 학술논문

桑树乳汁在桑树的生长和防御过程中起重要作用,乳汁中的糖苷酶抑制剂还是治疗糖尿病的有效成分.通过桑树cDNA文库中的序列比对,发现一段与乳汁合成相关的基因片段,采用RACE方法获得该基因的全长序列,基因cDNA全长1 145 bp,编码区759 bp,编码252个氨基酸残基,将该基因命名为桑树橡胶延伸因子基因(GenBank登录号:GQ466080).为探究该基因的功能,通过RT-PCR的方法分析该基因在桑树不同组织器官的表达,结果表明该基因在桑树幼叶中的表达量最高,其次是茎和芽,在根中的表达量最低.对桑树叶片进行细胞分裂素处理,发现细胞分裂素处理后该基因表达量有减少的趋势,在细胞分裂素处理的叶片中,正在伸展的幼叶中该基因的表达量要高于刚出现的幼叶和成熟叶.与桑树中已知的其它功能基因相比,桑树橡胶延伸因子在桑树正常生长中的表达量远高于桑树Na~+/H~+逆向转运蛋白基因MaNHX和桑树抗冻蛋白基因WAP27的表达量.从以上结果初步推测桑树橡胶延伸因子基因在桑树的生长发育中起重要作用.
상수유즙재상수적생장화방어과정중기중요작용,유즙중적당감매억제제환시치료당뇨병적유효성분.통과상수cDNA문고중적서렬비대,발현일단여유즙합성상관적기인편단,채용RACE방법획득해기인적전장서렬,기인cDNA전장1 145 bp,편마구759 bp,편마252개안기산잔기,장해기인명명위상수상효연신인자기인(GenBank등록호:GQ466080).위탐구해기인적공능,통과RT-PCR적방법분석해기인재상수불동조직기관적표체,결과표명해기인재상수유협중적표체량최고,기차시경화아,재근중적표체량최저.대상수협편진행세포분렬소처리,발현세포분렬소처리후해기인표체량유감소적추세,재세포분렬소처리적협편중,정재신전적유협중해기인적표체량요고우강출현적유협화성숙협.여상수중이지적기타공능기인상비,상수상효연신인자재상수정상생장중적표체량원고우상수Na~+/H~+역향전운단백기인MaNHX화상수항동단백기인WAP27적표체량.종이상결과초보추측상수상효연신인자기인재상수적생장발육중기중요작용.
Mulberry latex plays an important role in mulberry growth and defense. In addition, the glycosidase inhibitor in it is an active ingredient of antidiabetes. In a mulberry cDNA library, a gene fragment which is related to latex synthesis was identified through sequence alignment. Its full-length cDNA sequence was obtained by means of RACE and was designated as mulberry rubber elongation factor gene (MaREF, GenBank accession number GQ466080). It is 1145 bp long and has an open reading frame of 759 bp which encodes 252 amino acid residues. Expression analyses of MaREF gene in various mulberry tissues and organs through RT-PCR indicated that it had the highest expression level in young leaves, the moderate levels in stems and buds, and the lowest level in root. Treatment of mulberry leaves with cytokinin could reduce the expression level of MaREF gene. Among the cytokinin treated leaves, the expression level in young leaves at the expanding stage was higher than the newly emerged young leaves and mature leaves. Compared with other functional genes in mulberry, the expression level of MaREF gene was far much higher than the levels of mulberry Na~+/H~+ antiporter gene MaNHX and mulberry cold acclimation gene WAP27. The above results preliminarily suggest that MaREF gene plays an important role in mulberry growth and development.