CAJ | 학술논문

采用简单序列重复区间扩增ISSR分子标记技术对分布于四川省成都市的紫茉莉(Mirabilis jalapa L.)3种不同花色群体进行了遗传变异分析.从100条引物筛选出5条引物进行PCR扩增,共扩增出67条清晰条带,其中62条条带具多态性.紫茉莉的等位多态性位点百分数(PPB)为92.54%,观测等位基因数(Na)为1.925 4,有效等位基因数(Ne)为1.235 4,Nei的基因多样性指数(H)为0.156 2,shannon 指数(I)为0.263 3.3种花色的遗传一致度在0.905 4～0.962 0之间,遗传距离在0.038 8～0.099 4之间,其中白色与黄色的遗传距离最小(0.038 8),表现出最近的亲缘关系,黄色与紫色的遗传距离稍大(0.099 4),亲缘关系稍远.紫茉莉的遗传变异主要存在于群体内,不同花色群体间的基因流(Nm)为1.279.
채용간단서렬중복구간확증ISSR분자표기기술대분포우사천성성도시적자말리(Mirabilis jalapa L.)3충불동화색군체진행료유전변이분석.종100조인물사선출5조인물진행PCR확증,공확증출67조청석조대,기중62조조대구다태성.자말리적등위다태성위점백분수(PPB)위92.54%,관측등위기인수(Na)위1.925 4,유효등위기인수(Ne)위1.235 4,Nei적기인다양성지수(H)위0.156 2,shannon 지수(I)위0.263 3.3충화색적유전일치도재0.905 4～0.962 0지간,유전거리재0.038 8～0.099 4지간,기중백색여황색적유전거리최소(0.038 8),표현출최근적친연관계,황색여자색적유전거리초대(0.099 4),친연관계초원.자말리적유전변이주요존재우군체내,불동화색군체간적기인류(Nm)위1.279.
Mirabilis jalapa L. belong to the Mirabilis genus, which was classified in the family Nyctaginaceae. Inter-simple sequences repeats (ISSR) markers were used to analysis the genetic variation among 3 colonys with different color flowers. Of the 100 primers, 5 produced highly reproducible ISSR bands. These primers were carried out on 3 colonys of M. jalapa with purple, white and yellow flowers from Chengdu City, Sichuan Province. High genetic variation was revealed. 67discernable DNA fragments were with 62 (92. 54 % ) being polymorphic. Observed number of alleles ( Na ) , effective number of alleles ( Ne ) ,Nei's gene diversity ( H ) and Shannon's information index ( I ) was 1. 925 4,1. 235 4, 0. 156 2 and 0. 263 3, respectively. The values of genetic identity range from 0. 633 0 to 0. 908 0. Nei's genetic distance in the 3 colonys was from 0.038 8 to 0.099 4, which was the closest(0. 038 8) between colony with white flower and colony with yellow flower, and the farthest (0. 099 4) between colony with yellow flower and colony with purple flower. The genetic variation mainly occurred in colony of M. jalapa with same color flower, and Nm of different color populations was 1.279.