CAJ | 학술논문

用具有良好产黏活性的瑞士乳杆菌SR288与产酸力强的保加利亚乳杆菌NR902已灭活的原生质体电融合,构建高活性乳酸菌菌株.研究表明最佳电融合参数为:交变电场强度300V/cm,脉冲强度6kV/cm,脉冲时间40μs,脉冲个数4个.融合菌株能连续稳定遗传10代,生物活性检测表明,其产酸能力、表观黏度、持水力均优于亲株.
용구유량호산점활성적서사유간균SR288여산산력강적보가리아유간균NR902이멸활적원생질체전융합,구건고활성유산균균주.연구표명최가전융합삼수위:교변전장강도300V/cm,맥충강도6kV/cm,맥충시간40μs,맥충개수4개.융합균주능련속은정유전10대,생물활성검측표명,기산산능력、표관점도、지수력균우우친주.
A Lactobacillus fusant with high activity was constructed through electro-fusion between L. helveticus SR288 with slim-producing activity and L. bulgaricus NR902 with acid-producing activity. Results indicated that the optimal electro-fusion parameters were alternative electric field intensity 300 V/cm, impulse intensity 6 kV/cm, impulse time 40 μ s, and impulse number 4. The obtained fusion strain revealed excellent genetic stability up to 10 generations. Biological activity tests suggested that the fusant was superior to parental swains in acid-producing activity, apparent viscosity, and water-holding capacity.