CAJ | 학술논문

对7种乳酸菌的16S rRNA基因的部分序列进行了PCR扩增和序列测定,进行序列比对后,以清酒乳杆菌为外组群.运用MEGA2.0和Tree-Przz5.0遗传分析软件以邻接法(NJ)、最大简约法(MP)和最大似然法(ML)构建系统发生树.结果表明:4种乳酸菌(类干酪乳杆菌、干酪乳杆菌、鼠李糖乳杆菌、戊糖乳杆菌)为一支系,另外3种(类植物乳杆菌、弯曲乳杆菌、草乳杆菌)构成另一支系,这两个支系又互为姊妹群.结果还显示,乳酸菌高级分类单元(超科及以上分类阶元)的系统发生与现行形态分类体系间存在明显的分歧.
대7충유산균적16S rRNA기인적부분서렬진행료PCR확증화서렬측정,진행서렬비대후,이청주유간균위외조군.운용MEGA2.0화Tree-Przz5.0유전분석연건이린접법(NJ)、최대간약법(MP)화최대사연법(ML)구건계통발생수.결과표명:4충유산균(류간락유간균、간락유간균、서리당유간균、무당유간균)위일지계,령외3충(류식물유간균、만곡유간균、초유간균)구성령일지계,저량개지계우호위자매군.결과환현시,유산균고급분류단원(초과급이상분류계원)적계통발생여현행형태분류체계간존재명현적분기.
The partial sequence of 16S rRNA gene from seven species of lactic acid bacteria commonly found in wine were amplified through the poly merase chain reaction(PCR)and the PCR products were sequenced directly with ABI-377 DNA automatic sequencer.L sake as an outgroup.and we constructed that their molecular phylogenetic trees with neighbour-joining (NJ)、maximum parsimony(MP)and maximum likelihood(ML)methods using MEGA(Version 2.0)and Tree-Puzzle(Version 5.0) software.The results showed that the four species of lactic acid bacteria(L.paracasei,L.casei,L.rhamrgosus,L.pentosus) constituted a monophyletic group;another three species of lactic acid bacteria (L.paraplantarum,L.cutvatus,L.graminis)also made up a polyphyletic group.Furthermore,the two sister groups were monophyletic.That the molecular phylogenies of the higher taxonomic groups(above superfamily)were mostly in conflict with the morphological ones was also showed by the results.