国际输血及血液学杂志
國際輸血及血液學雜誌
국제수혈급혈액학잡지
INTERNATIONAL JOURNAL OF BLOOD TRANSFUSION AND HEMATOLOGY
2011年
6期
486-488
,共3页
孔亚红%王朔桂%秦盛峰%牟敏红
孔亞紅%王朔桂%秦盛峰%牟敏紅
공아홍%왕삭계%진성봉%모민홍
艾滋病%凝聚胺法%微柱凝胶法%不规则抗体
艾滋病%凝聚胺法%微柱凝膠法%不規則抗體
애자병%응취알법%미주응효법%불규칙항체
AIDS%polybrene method%micro-column gel%irregular antibodies
目的 探讨艾滋病(AIDS)患者不规则抗体类型及性质,对两种交叉配血方法结果的影响.方法 选择2010年1月至12月在本院住院期间,需输血治疗的387例AIDS患者为研究对象.对其分别采用微柱凝胶法、凝聚胺法进行交叉配血及不规则抗体筛查实验(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准,得到该委员会批准,征得受试者知情同意,并与之签署临床研究知情同意书).观察患者不规则抗体阳性率、交叉配血不相合率、两种配血方法的敏感度及不规则抗体类型和性质.结果 ①微柱凝胶法检出患者不规则抗体为182例,配血不相合率为47.03％(182/387);凝聚胺法检出患者不规则抗体为11例,配血不相合率为2.84％(11/387);②两种配血方法敏感度比较,差异有统计学意义(x2=9.6,P＜0.005);③患者不规则抗体性质为低亲和力小分子抗体,属于抗IgG+ C3d+ IgA+ IgM型和/或抗IgG、C3d、IgA和IgM型.结论 由于AIDS患者不规则抗体类型及性质不同,因此,对两种配血方法结果的影响程度亦不相同.
目的 探討艾滋病(AIDS)患者不規則抗體類型及性質,對兩種交扠配血方法結果的影響.方法 選擇2010年1月至12月在本院住院期間,需輸血治療的387例AIDS患者為研究對象.對其分彆採用微柱凝膠法、凝聚胺法進行交扠配血及不規則抗體篩查實驗(本研究遵循的程序符閤本院人體試驗委員會所製定的倫理學標準,得到該委員會批準,徵得受試者知情同意,併與之籤署臨床研究知情同意書).觀察患者不規則抗體暘性率、交扠配血不相閤率、兩種配血方法的敏感度及不規則抗體類型和性質.結果 ①微柱凝膠法檢齣患者不規則抗體為182例,配血不相閤率為47.03％(182/387);凝聚胺法檢齣患者不規則抗體為11例,配血不相閤率為2.84％(11/387);②兩種配血方法敏感度比較,差異有統計學意義(x2=9.6,P＜0.005);③患者不規則抗體性質為低親和力小分子抗體,屬于抗IgG+ C3d+ IgA+ IgM型和/或抗IgG、C3d、IgA和IgM型.結論 由于AIDS患者不規則抗體類型及性質不同,因此,對兩種配血方法結果的影響程度亦不相同.
목적 탐토애자병(AIDS)환자불규칙항체류형급성질,대량충교차배혈방법결과적영향.방법 선택2010년1월지12월재본원주원기간,수수혈치료적387례AIDS환자위연구대상.대기분별채용미주응효법、응취알법진행교차배혈급불규칙항체사사실험(본연구준순적정서부합본원인체시험위원회소제정적윤리학표준,득도해위원회비준,정득수시자지정동의,병여지첨서림상연구지정동의서).관찰환자불규칙항체양성솔、교차배혈불상합솔、량충배혈방법적민감도급불규칙항체류형화성질.결과 ①미주응효법검출환자불규칙항체위182례,배혈불상합솔위47.03％(182/387);응취알법검출환자불규칙항체위11례,배혈불상합솔위2.84％(11/387);②량충배혈방법민감도비교,차이유통계학의의(x2=9.6,P＜0.005);③환자불규칙항체성질위저친화력소분자항체,속우항IgG+ C3d+ IgA+ IgM형화/혹항IgG、C3d、IgA화IgM형.결론 유우AIDS환자불규칙항체류형급성질불동,인차,대량충배혈방법결과적영향정도역불상동.
Objective To investigate the influence of the irregular antibody types and properties of AIDS patients to the results of two kinds of blood cross matching methods.Method From January to December 2010,387 cases of AIDS patients who received blood transfusion in Nanning Fourth People's Hospital were included in this study.Polybrene and micro-column gel methods were used in blood cross matching and irregular antibody screening test,respectively.The irregular antibody positive rate,blood cross matching mismatched rate,the sensitivity of these two kinds of matching methods,and irregular antibody types and properties were observed.The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Nanning Fourth People′s Hospital.Informed consents were obtained from all participants.Results ① 182 cases with irregular antibodies were detected by micro column gel method with the non-consistent rate of 47.03 ％(182/387).11 cases with irregular antibodies were detected by polybrene method with non-consistent rate of 2.84％(11/387).② The sensitivity was significant difference between two kinds of matching methods(x2 =9.6,P＜0.005).③Irregular antibodies were low affinity,small molecular antibodies with the type of IgG+C3d+ IgA+,IgM,or anti-IgG,anti-C3d,antiIgA,and anti-IgM.Conclusion Because of the different types and properties of irregular antibody of AIDS patients,the results of two kinds of matching methods were not the same.
