CAJ | 학술논문

目的从分子水平探索细胞因子治疗急性放射病的作用机制.方法 4.5 Gy γ射线照射后收集不同时间点细胞因子治疗组比格犬血清,应用二维电泳分离血清中差异表达的蛋白质,找出其中差异显著的蛋白质点进行质谱分析,并确定蛋白质的氨基酸序列.结果获得了分辨率较高的凝胶蛋白图谱.共筛选出6个差异表达蛋白质点,成功鉴定出其中的4个蛋白质点.经氨基酸序列分析发现其中一个较有意义的蛋白质是相对分子质量为13 077、等电点为6.26的血清淀粉样蛋白A(SAA).该蛋白在照射前及照后36 d均无表达,照射后1 d略有增强(吸光度为0.2166),14 d显著增强(吸光度为0.4577).结论 SAA的表达变化与病程进展相一致,可能对病情的转归有重要的提示意义.
목적 종분자수평탐색세포인자치료급성방사병적작용궤제.방법 4.5 Gy γ사선조사후수집불동시간점세포인자치료조비격견혈청,응용이유전영분리혈청중차이표체적단백질,조출기중차이현저적단백질점진행질보분석,병학정단백질적안기산서렬.결과 획득료분변솔교고적응효단백도보.공사선출6개차이표체단백질점,성공감정출기중적4개단백질점.경안기산서렬분석발현기중일개교유의의적단백질시상대분자질량위13 077、등전점위6.26적혈청정분양단백A(SAA).해단백재조사전급조후36 d균무표체,조사후1 d략유증강(흡광도위0.2166),14 d현저증강(흡광도위0.4577).결론 SAA적표체변화여병정진전상일치,가능대병정적전귀유중요적제시의의.
Objective To explore the mechanisms of cytokines on acute radiation disease in irradiated beagles.Methods The sera of beagles irradiated with 4.5 Gy γ-rays with cytokines treatment was collected at different time points post irradiation.The two-dimensional gel electrophoresis(2-DE)was used to isolate and compare the differentially expressed proteins in sera.HD-MS was used to analyze the differentially expressed proteins with significance,and the amino acid sequences should be determined. Results High resolution 2-DE gel map was obtained.There were six differentially expressed proteins in sera of irradiated beagles at different time points.Four protein spots were successfully identified by MS.A significant spot was identified as serum amyloid A(SAA)by HD-MS,with relative molecular mass of 13 077 and isoelectfie point of 6.26.Expression of SAA was not found 1 d pre-irradiation and 36 d postirradiation,but increased slightly 1 d(0.2166)and significantly 14 d post-irradiation(0.4577). Conclusions The expression of serum amyloid A was consistent with the process of acute radiation injury,which might indicate the turnover of the disease.