国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2011年
3期
187-189
,共3页
铜绿假单胞菌%氧浓度%生物被膜
銅綠假單胞菌%氧濃度%生物被膜
동록가단포균%양농도%생물피막
Pseudomonas aeruginosa%Oxygen concentration%Biofilm
目的 观察氧浓度变化对铜绿假单胞菌生物被膜产生量的影响,对存在铜绿假单胞菌感染高危因素的患者是否适合高浓度的氧疗做出分析.方法 10株铜绿假单胞菌临床分离株(均来源于不同克隆株)分别在无氧(0%)、低氧(10%)、正常氧(20%)、高氧(30%、40%、50%、60%)7个不同的氧浓度下连续培养3 d后,分别用结晶紫染色法检测生物被膜产生量;硝酸银染色法观察不同氧浓度环境下生物被膜的重要成分胞外多糖产生的时间窗.结果 氧浓度为50%时生物被膜产生量达到最高值(A570=1.61),趋势性分析表明其产生量均随着氧浓度的升高而上升,相关性分析显示其产生量与氧浓度呈正相关(R=0.59).胞外多糖的检测结果显示氧浓度大于40%时,菌株培养1 h镜下即可见到银染成黑色的胞外多糖;氧浓度为20%、30%时,所需时间为2 h;10%氧浓度需要4 h;无氧条件下需要8h,即随着氧浓度的升高细菌出现胞外多糖的时间窗越短.结论 体外实验中铜绿假单胞菌在高氧环境下更容易产生生物被膜.
目的 觀察氧濃度變化對銅綠假單胞菌生物被膜產生量的影響,對存在銅綠假單胞菌感染高危因素的患者是否適閤高濃度的氧療做齣分析.方法 10株銅綠假單胞菌臨床分離株(均來源于不同剋隆株)分彆在無氧(0%)、低氧(10%)、正常氧(20%)、高氧(30%、40%、50%、60%)7箇不同的氧濃度下連續培養3 d後,分彆用結晶紫染色法檢測生物被膜產生量;硝痠銀染色法觀察不同氧濃度環境下生物被膜的重要成分胞外多糖產生的時間窗.結果 氧濃度為50%時生物被膜產生量達到最高值(A570=1.61),趨勢性分析錶明其產生量均隨著氧濃度的升高而上升,相關性分析顯示其產生量與氧濃度呈正相關(R=0.59).胞外多糖的檢測結果顯示氧濃度大于40%時,菌株培養1 h鏡下即可見到銀染成黑色的胞外多糖;氧濃度為20%、30%時,所需時間為2 h;10%氧濃度需要4 h;無氧條件下需要8h,即隨著氧濃度的升高細菌齣現胞外多糖的時間窗越短.結論 體外實驗中銅綠假單胞菌在高氧環境下更容易產生生物被膜.
목적 관찰양농도변화대동록가단포균생물피막산생량적영향,대존재동록가단포균감염고위인소적환자시부괄합고농도적양료주출분석.방법 10주동록가단포균림상분리주(균래원우불동극륭주)분별재무양(0%)、저양(10%)、정상양(20%)、고양(30%、40%、50%、60%)7개불동적양농도하련속배양3 d후,분별용결정자염색법검측생물피막산생량;초산은염색법관찰불동양농도배경하생물피막적중요성분포외다당산생적시간창.결과 양농도위50%시생물피막산생량체도최고치(A570=1.61),추세성분석표명기산생량균수착양농도적승고이상승,상관성분석현시기산생량여양농도정정상관(R=0.59).포외다당적검측결과현시양농도대우40%시,균주배양1 h경하즉가견도은염성흑색적포외다당;양농도위20%、30%시,소수시간위2 h;10%양농도수요4 h;무양조건하수요8h,즉수착양농도적승고세균출현포외다당적시간창월단.결론 체외실험중동록가단포균재고양배경하경용역산생생물피막.
Objective The aims of this study were to investigate the relationship between oxygen concentration and biofilm production of P. aeruginosa. Methods Ten genetically different clinieal strains of P. aeruginosa were cultured for 3 days at different levels of environmental oxygen such as no oxygen (0%), low oxygen (10%), normal oxygen (20%) and high oxygen (30%, 40%, 50%, 60%),respcetively. Then biofilm mass was quantified by crystal violet dye, silver nitrate staning to determine the time window of extra polysaccharide production under different levels of environmental oxygen. Results For quantifying the amount of biofilm mass, the highest amount of biofilm mass (1.61) was produced when the oxygen concentration was 50 %. Linear correlation analysis indicated that a significant positive correlation existed between the oxygen concentration and biofilm mass produetion (R = 0. 59). The production of extra polysaccharide by P. aeruginosa was not visualized until culture for 1 hour at ＞40%environmental oxygen concentration,2 hours for 20% and 30% oxygen concentrations, 4 hours for 10 %oxygen concentration, and 8 hours for anaerobic conditions. The time window of extra polysaccharide production was shorter with the increase of oxygen. Conclusions Hyperoxia promoted the ability of producing biofilm of P. aeruginosa which is in favor of resistance and colonization.
