CAJ | 학술논문

目的探讨多沙普仑对七氟醚抑制大鼠延髓呼吸中枢兴奋性的影响.方法出生1～4d的SD大鼠,雄雌不拘,制备离体延髓-脊髓标本,置于95％ O2-5％CO2饱和的人工脑脊液中.采用随机数字表法,将其随机分为3组(n=9):对照组(C组)、七氟醚组(S组)和七氟醚+多沙普仑组(S+D组).采用生物信号采集和处理系统记录舌下神经根呼吸节律放电.稳定10 min后,C组继续给予人工脑脊液灌流10 min;S组灌流5％七氟醚10 min;S+D组灌流5％七氟醚和5 μmol/L多沙普仑的混合液10 min.记录呼吸周期、吸气时程和吸气放电积分幅度.结果与C组比较,S组呼吸周期延长,吸气时间缩短,吸气放电积分幅度降低(P＜0.05),S+D组上述各指标差异无统计学意义(P＞0.05)；与S组比较,S+D组呼吸周期缩短,吸气时间延长,吸气放电积分幅度升高(P＜0.05).结论多沙普仑可拮抗七氟醚对延髓呼吸中枢兴奋性的抑制作用.
목적 탐토다사보륜대칠불미억제대서연수호흡중추흥강성적영향.방법 출생1～4d적SD대서,웅자불구,제비리체연수-척수표본,치우95％ O2-5％CO2포화적인공뇌척액중.채용수궤수자표법,장기수궤분위3조(n=9):대조조(C조)、칠불미조(S조)화칠불미+다사보륜조(S+D조).채용생물신호채집화처리계통기록설하신경근호흡절률방전.은정10 min후,C조계속급여인공뇌척액관류10 min;S조관류5％칠불미10 min;S+D조관류5％칠불미화5 μmol/L다사보륜적혼합액10 min.기록호흡주기、흡기시정화흡기방전적분폭도.결과 여C조비교,S조호흡주기연장,흡기시간축단,흡기방전적분폭도강저(P＜0.05),S+D조상술각지표차이무통계학의의(P＞0.05)；여S조비교,S+D조호흡주기축단,흡기시간연장,흡기방전적분폭도승고(P＜0.05).결론 다사보륜가길항칠불미대연수호흡중추흥강성적억제작용.
Objective To investigate the effect of doxapram on inhibition of medullary respiratory center excitability by sevoflurane in rats.Methods Neonatal Sprague-Dawley rats of both sexes,aged 1-4 days,were used in this study.Isolated medulla oblongata-spinal cord specimens were made according to the method described by Suzue and perfused with the artificial cerebrospinal fluid saturated with 95％O2-5％CO2.The specimens were randomly divided into 3 groups ( n =9 each):control group (group C),sevoflurane group (group S) and sevoflurane + doxapram group (group S + D).Respiratory rhythmical discharge activity of the hypoglossal nerve rootlets was recorded using suction electrode.After 10 min of equilibration,the specimens were perfused with the artificial cerebrospinal fluid,5％ sevoflurane and the mixture of 5％ sevoflurane and 5 μmol/L doxapram for 10 min in groups C,S,and S + D respectively.The respiratory cycle,inspiratory time and integral amplitude of inspiratory discharge were recorded.Results Compared with group C,the respiratory cycle was significantly prolonged,the inspiratory time was significantly shortened,and the integral amplitude of inspiratory discharge was significantly decreased in group S (P ＜ 0.05),and no significant change was found in the parameters mentioned above in group S + D (P ＞ 0.05).Compared with group S,the respiratory cycle was significantly shortened,the inspiratory time was significantly prolonged,the integral amplitude of inspiratory discharge was significantly increased in group S + D ( P ＜ 0.05).Conclusion Doxapram antagonizes sevoflurane-induced inhibition of excitability of medullary respiratory center in rats.