CAJ | 학술논문

目的观察雷公藤内酯醇(TRI)对大鼠肾缺血再灌注损伤时肾组织中Toll样受体4(TLR4)表达的影响.方法随机将51只Wistar大鼠分为3组.(1)阴性对照组(n=15):游离双侧肾脏,切除右肾,缝合腹壁.(2)缺血再灌注组(n=18):实验过程与阴性对照组相同,但在切除右肾和游离左肾之后,将左肾动、静脉夹闭45 min,然后开通.(3)TRI处理组(n=18):肾缺血再灌注前3 d经大鼠腹腔注射TRI 0.4 mg/kg,每天1次,连续3 d,其他实验过程与缺血再灌注组相同.肾缺血再灌注1、3、5 d后,分别采用全自动生化分析仪检测血清尿素氮(BUN)和肌酐(Cr)的含量;逆转录聚合酶链反应(RT-PCR)方法检测肾组织中TLR4 mRNA的表达水平;免疫印记法(Western blot)检测肾组织中TLR4表达水平.结果肾缺血再灌注1、3、5 d后,缺血再灌注组和TRI处理组血清BUN及Cr均明显高于阴性对照组(P＜0.01),肾组织中TLR4 mRNA和TLR4的表达也明显高于阴性对照组(P＜0.05);但与缺血再灌注组比较,TRI处理组血清BUN和Cr明显降低(P＜0.01),肾组织中TLR4 mRNA和TLR4的表达也显著降低(P＜0.05).结论雷公藤内酯醇可以减轻肾缺血再灌注损伤,其机制可能是通过抑制TLR4的表达而发挥作用的.
목적 관찰뢰공등내지순(TRI)대대서신결혈재관주손상시신조직중Toll양수체4(TLR4)표체적영향.방법 수궤장51지Wistar대서분위3조.(1)음성대조조(n=15):유리쌍측신장,절제우신,봉합복벽.(2)결혈재관주조(n=18):실험과정여음성대조조상동,단재절제우신화유리좌신지후,장좌신동、정맥협폐45 min,연후개통.(3)TRI처리조(n=18):신결혈재관주전3 d경대서복강주사TRI 0.4 mg/kg,매천1차,련속3 d,기타실험과정여결혈재관주조상동.신결혈재관주1、3、5 d후,분별채용전자동생화분석의검측혈청뇨소담(BUN)화기항(Cr)적함량;역전록취합매련반응(RT-PCR)방법검측신조직중TLR4 mRNA적표체수평;면역인기법(Western blot)검측신조직중TLR4표체수평.결과 신결혈재관주1、3、5 d후,결혈재관주조화TRI처리조혈청BUN급Cr균명현고우음성대조조(P＜0.01),신조직중TLR4 mRNA화TLR4적표체야명현고우음성대조조(P＜0.05);단여결혈재관주조비교,TRI처리조혈청BUN화Cr명현강저(P＜0.01),신조직중TLR4 mRNA화TLR4적표체야현저강저(P＜0.05).결론 뢰공등내지순가이감경신결혈재관주손상,기궤제가능시통과억제TLR4적표체이발휘작용적.
Objective To observe the effects of Triptolide on the expression of toll-like receptor 4 (TLR4) in renal ischemia/reperfusion (I/R) injury in rats. Methods A renal I/R model was established. Rats were randomly separated into the following experimental groups. Group 1, shamoperated control (n = 15) : rats were subjected to surgical manipulation, without the induction of renal ischemia. Group 2, I/R (n = 18): rats were subjected to left renal ischemia for 45 min followed by reperfusion. Group 3, TRI + I/R (n = 18): Before the I/R procedure (as in group 2), rats were intraperitoneally injected with TRI (0.4 mg/kg), once every day, three times. Rats were killed at the 1st, 3rd, and 5th day after I/R injury. The parameters of renal function were determined by autobiochemical analyzer. The expression of TLR4 was detected by RT-PCR and Western blotting. Results As comparedwith the sham-operated control group, serum BUN and Cr levels were significantly increased in the rats undergoing I/R procedure at the 1st, 3rd, and 5th day (P＜0. 01). After the treatment with TRI, the levels of BUN and Cr and the expression of TLR4 in the renal tissues were significantly decreased (P＜0. 05). Conclusion TRI could relieve renal I/R injury in rats by inhibiting the TLR4 expression.