中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2008年
11期
1517-1519
,共3页
易清平%刘小孙%王华曦%陈荣军%卢峡%容松
易清平%劉小孫%王華晞%陳榮軍%盧峽%容鬆
역청평%류소손%왕화희%진영군%로협%용송
肾%移植%显微手术%模型,动物
腎%移植%顯微手術%模型,動物
신%이식%현미수술%모형,동물
Kidney%Transplantation%Microsurgery%Model,animal
目的 建立更简便的小鼠肾移植模型,方便术者快速掌握此技术.方法 采用C57BL/6小鼠行20例同种同体异位肾移植,将供体连接肾动脉的腹主动脉袖套和肾静脉分别与受体腹主动脉及下腔静脉行端侧吻合,每侧壁缝针数分别为2~3针及4~6针,线尾不用打结.输尿管重建采用改良法将供体输尿管末端游离于膀胱内加外固定.结果 总手术时间为(45.1±3.5)min,首次手术成功率为95%,模型成功率85%,术后观察12周,血清肌酐水平平稳正常,体重增加,肾组织学无明显异常.结论 该模型简单易行,容易控制吻合口大小防止吻合口血栓及出血,是理想的手术模型.
目的 建立更簡便的小鼠腎移植模型,方便術者快速掌握此技術.方法 採用C57BL/6小鼠行20例同種同體異位腎移植,將供體連接腎動脈的腹主動脈袖套和腎靜脈分彆與受體腹主動脈及下腔靜脈行耑側吻閤,每側壁縫針數分彆為2~3針及4~6針,線尾不用打結.輸尿管重建採用改良法將供體輸尿管末耑遊離于膀胱內加外固定.結果 總手術時間為(45.1±3.5)min,首次手術成功率為95%,模型成功率85%,術後觀察12週,血清肌酐水平平穩正常,體重增加,腎組織學無明顯異常.結論 該模型簡單易行,容易控製吻閤口大小防止吻閤口血栓及齣血,是理想的手術模型.
목적 건립경간편적소서신이식모형,방편술자쾌속장악차기술.방법 채용C57BL/6소서행20례동충동체이위신이식,장공체련접신동맥적복주동맥수투화신정맥분별여수체복주동맥급하강정맥행단측문합,매측벽봉침수분별위2~3침급4~6침,선미불용타결.수뇨관중건채용개량법장공체수뇨관말단유리우방광내가외고정.결과 총수술시간위(45.1±3.5)min,수차수술성공솔위95%,모형성공솔85%,술후관찰12주,혈청기항수평평은정상,체중증가,신조직학무명현이상.결론 해모형간단역행,용역공제문합구대소방지문합구혈전급출혈,시이상적수술모형.
Objective To establish a model of kidney transplantation in mice more convenient. Methods Twenty C57BL/6 mice were used for kidney isografts. The end-to-side anastomosis was per-formed between the donor aortic cuff with renal artery and recipient aorta of abdomen, and the donor renal vein and recipient IVC. 2-3 stitches and 4-6 stitches were required for each side' s anastomosis. It didn't need to be tied at the end of the suture. The construct of ureter was modified with joining the donor ureter to the recipient bladder. Results Total operative time was (45.1±3.5) min. The success rate was 95%. All mice were observed for 12 weeks after operation. Serum creatinine level was in normal range. Body weight gain was observed. Renal histology was normal. Conclusion This model is easy and conven- ient. The anastomosis can be controlled to prevent thrombosis and bleeding. It is an ideal model.