中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2011年
7期
460-464
,共5页
胰腺炎,急性坏死性%高脂血症%胃肠活动%氮能神经元%胆碱能纤维%疾病模型,动物
胰腺炎,急性壞死性%高脂血癥%胃腸活動%氮能神經元%膽堿能纖維%疾病模型,動物
이선염,급성배사성%고지혈증%위장활동%담능신경원%담감능섬유%질병모형,동물
Pancreatitis,acute%Hyperlipidemias%Gastrointestinal metility%Nitriergic neurons%Cholinergic fibers%Disease models,animal
目的 探讨肠肌间神经丛形态改变与伴高脂血症急性坏死性胰腺炎(ANP)大鼠胃肠传输延迟的关系.方法 将40只雄性SD大鼠均分为4组(正常对照组、单纯ANP组、高脂对照组、高脂ANP组),分组采用高脂饲料喂养4周建立大鼠高脂血症模型,应用逆行胰胆管注射3.5%牛磺胆酸钠制作ANP大鼠模型.用墨汁灌胃法测量各组大鼠胃肠传输距离,计算胃肠传输率,以Karnovsky-Root直接法和NADPH组织化学法观察回肠肌间神经丛胆碱能和氮能神经的组织形态学改变.结果 高脂ANP组较单纯ANP组胰腺炎病理损伤加重(病理学评分12.8±0.63比10.8±1.93,P<0.01),胃肠传输明显延迟(传输率27%±5%比38%±6%,P<0.01),胆碱能神经元密度下降(4.80±1.23比5.80±0.79,P<0.05),氮能神经元密度明显升高(8.70±0.75比6.80±1.48,P<0.01).肌间神经丛乙酰胆碱脂酶和一氧化氮合酶改变与胃肠传输率有线性回归关系(R2=0.531,P<0.01).结论 伴高脂血症ANP的大鼠存在明显胃肠动力障碍,肠肌间神经丛形态改变与其密切相关.
目的 探討腸肌間神經叢形態改變與伴高脂血癥急性壞死性胰腺炎(ANP)大鼠胃腸傳輸延遲的關繫.方法 將40隻雄性SD大鼠均分為4組(正常對照組、單純ANP組、高脂對照組、高脂ANP組),分組採用高脂飼料餵養4週建立大鼠高脂血癥模型,應用逆行胰膽管註射3.5%牛磺膽痠鈉製作ANP大鼠模型.用墨汁灌胃法測量各組大鼠胃腸傳輸距離,計算胃腸傳輸率,以Karnovsky-Root直接法和NADPH組織化學法觀察迴腸肌間神經叢膽堿能和氮能神經的組織形態學改變.結果 高脂ANP組較單純ANP組胰腺炎病理損傷加重(病理學評分12.8±0.63比10.8±1.93,P<0.01),胃腸傳輸明顯延遲(傳輸率27%±5%比38%±6%,P<0.01),膽堿能神經元密度下降(4.80±1.23比5.80±0.79,P<0.05),氮能神經元密度明顯升高(8.70±0.75比6.80±1.48,P<0.01).肌間神經叢乙酰膽堿脂酶和一氧化氮閤酶改變與胃腸傳輸率有線性迴歸關繫(R2=0.531,P<0.01).結論 伴高脂血癥ANP的大鼠存在明顯胃腸動力障礙,腸肌間神經叢形態改變與其密切相關.
목적 탐토장기간신경총형태개변여반고지혈증급성배사성이선염(ANP)대서위장전수연지적관계.방법 장40지웅성SD대서균분위4조(정상대조조、단순ANP조、고지대조조、고지ANP조),분조채용고지사료위양4주건립대서고지혈증모형,응용역행이담관주사3.5%우광담산납제작ANP대서모형.용묵즙관위법측량각조대서위장전수거리,계산위장전수솔,이Karnovsky-Root직접법화NADPH조직화학법관찰회장기간신경총담감능화담능신경적조직형태학개변.결과 고지ANP조교단순ANP조이선염병리손상가중(병이학평분12.8±0.63비10.8±1.93,P<0.01),위장전수명현연지(전수솔27%±5%비38%±6%,P<0.01),담감능신경원밀도하강(4.80±1.23비5.80±0.79,P<0.05),담능신경원밀도명현승고(8.70±0.75비6.80±1.48,P<0.01).기간신경총을선담감지매화일양화담합매개변여위장전수솔유선성회귀관계(R2=0.531,P<0.01).결론 반고지혈증ANP적대서존재명현위장동력장애,장기간신경총형태개변여기밀절상관.
Objective To explore association between the changes of myenteric nerve plexus and the delayed gastrointestinal transit in acute necrotic pancreatitis (ANP) rat complicated with hyperlipemia. Methods Atotal of 40 male Sprague-Dawley (SD) rats were randomly divided into 4 groups (normal control group, ANP group, hyperlipemia (HL) control group and HL with ANP group). HL rat model was established by fed with high-fat diet for 4 weeks, and ANP rat model was induced by retrograde injection of 3.5% sodium taurocholate into pancreatic duct. Gastrointestina1 transit distance was measured by ink gavage method. The histological changes of cholinergic and nitriergic nerves in myenteric plexus were observed by Karnovsky-Root method and NADPH histochemistry method. Results Pathological injuries were more severe in HL with ANP group than in ANP group (12.8±0.63 vs. 10.8±1.93,P<0.01), gastrointestinal transit was obviously delayed (transmission rate was 27%±5% vs. 38%±6%,P<0.01), the density of cholinergic neurons decreased (4.80±1.23 vs. 5.80±0.79, P<0.05), and the density of nitriergic neurons significantly increased (8.70±0.75 vs. 6.80±1.48, P<0.01). There was a linear regression between changes of cholinergic and nitrievgic nerves in myenteric nerve plexus and gastrointestinal transit (R2=0.531, P<0.01). Conclusion There was significant gastrointestinal motility disorder in the ANP rat complicated with hyperlipemia, which may be closely related with the changes of myenteric nerve plexus.
