植物学报
植物學報
식물학보
ACTA BOTANICA SINICA
2004年
11期
1383-1386
,共4页
赵昱%于荣敏%Cornelia SCHROEDER%Ian SADLER%Matthias UNGER%孙先凤%David W.H RANKIN%Joachim ST(O)CKIGT
趙昱%于榮敏%Cornelia SCHROEDER%Ian SADLER%Matthias UNGER%孫先鳳%David W.H RANKIN%Joachim ST(O)CKIGT
조욱%우영민%Cornelia SCHROEDER%Ian SADLER%Matthias UNGER%손선봉%David W.H RANKIN%Joachim ST(O)CKIGT
生物转化%蛇根木%细胞培养%紫杉醇%紫杉烷类
生物轉化%蛇根木%細胞培養%紫杉醇%紫杉烷類
생물전화%사근목%세포배양%자삼순%자삼완류
biotransformation%cell culture%Paclitaxel%taxanes%Rauwolfia serpentina
通过理化及光谱学方法,从蛇根木(Rautwolfia serpentina(L.)Benth.et Kurz.)悬浮细胞内分离到3个紫杉醇同系物,高分辨1H-NMR和MS结构分析表明,它们分别为10-脱乙酰紫杉醇、baccatinⅢ和10-deacetylbaccatinⅢ.本实验未检测出紫杉醇的苷化或羟基化衍生物.
通過理化及光譜學方法,從蛇根木(Rautwolfia serpentina(L.)Benth.et Kurz.)懸浮細胞內分離到3箇紫杉醇同繫物,高分辨1H-NMR和MS結構分析錶明,它們分彆為10-脫乙酰紫杉醇、baccatinⅢ和10-deacetylbaccatinⅢ.本實驗未檢測齣紫杉醇的苷化或羥基化衍生物.
통과이화급광보학방법,종사근목(Rautwolfia serpentina(L.)Benth.et Kurz.)현부세포내분리도3개자삼순동계물,고분변1H-NMR화MS결구분석표명,타문분별위10-탈을선자삼순、baccatinⅢ화10-deacetylbaccatinⅢ.본실험미검측출자삼순적감화혹간기화연생물.
The biotransformation of paclitaxel (Taxol ) by the cell suspension cultures of Rauwolfia serpentina (L.) Benth. et Kurz. were investigated. Three Paclitaxel-based intracellular metabolites were detected from the cell filter cake and were, by high field 1H-NMR and MS data, identified as 10-deacetyltaxol,baccatin Ⅲ, and 10-deacetylbaccatin Ⅲ. No glucosidated or hydroxylated derivatives were checked out in this incubation experiment.
