CAJ | 학술논문

[目的]为体外研制人参皂苷及其衍生物的抗MDV作用机制提供理论依据.[方法]采用中性红染料吸收法,检测了人参皂苷及其衍生物对鸡胚成纤维细胞(CEF)体外培养中增殖活性的影响.[结果]各药物的促增殖作用不完全相同,低毒性的药物促增殖作用更明显.从药物对CEF细胞的不同作用时间点来看,以72 h的OD值和正常对照的差异最大,在24 h和正常对照组差异不显著.[结论]人参皂苷及其衍生物在中、低浓度能够促进CEF细胞的增殖,且药物作用具有时间依赖性.
[목적]위체외연제인삼조감급기연생물적항MDV작용궤제제공이론의거.[방법]채용중성홍염료흡수법,검측료인삼조감급기연생물대계배성섬유세포(CEF)체외배양중증식활성적영향.[결과]각약물적촉증식작용불완전상동,저독성적약물촉증식작용경명현.종약물대CEF세포적불동작용시간점래간,이72 h적OD치화정상대조적차이최대,재24 h화정상대조조차이불현저.[결론]인삼조감급기연생물재중、저농도능구촉진CEF세포적증식,차약물작용구유시간의뢰성.
[Objective] The research aimed to provide theoretical basis for studying anti-MDV mechanism of ginsenoside and its derivatives in vitro. [Method] Effects of ginsenoside and its derivatives on proliferation activity of chick embryo fibroblast (CEF) in vitro were determined by using neutral red dye absorption method. [Result] The results showed the proliferation effects of different drugs are not completely same, and are more obvious with low toxic drugs. Seeing from different action time, the differences of OD value was biggest at 72 h when compared with normal control group, while there was no significant difference at 24 h. [Conclusion] Ginsenoside and its derivatives could promote the proliferation of CEF cells in medium and low concentrations, which have time-dependent characteristic.