CAJ | 학술논문

以百合新鲜鳞叶为材料,利用RAPD分子标记对来自我国5省共16份百合样品进行了分析.结果表明:采用改良CTAB法提取百合鳞叶中DNA条带清晰较,且从120条随机引物中筛选出35条有效引物,共得到769个扩增位点,其中628个位点具有多态性,占81.7%.聚类分析表明,16个百合材料在阈值为0.9407时分为2个RAPD群,即分别属于百合科的百合属和大百合属;阈值为0.5790时,百合属分为3个种,即分别为百合、卷丹和细叶百合.这些均可为进一步研究百合的分子生物学特性打下基础,为鉴定中药材百合品种品系的新方法提供充分的实验依据.
이백합신선린협위재료,이용RAPD분자표기대래자아국5성공16빈백합양품진행료분석.결과표명:채용개량CTAB법제취백합린협중DNA조대청석교,차종120조수궤인물중사선출35조유효인물,공득도769개확증위점,기중628개위점구유다태성,점81.7%.취류분석표명,16개백합재료재역치위0.9407시분위2개RAPD군,즉분별속우백합과적백합속화대백합속;역치위0.5790시,백합속분위3개충,즉분별위백합、권단화세협백합.저사균가위진일보연구백합적분자생물학특성타하기출,위감정중약재백합품충품계적신방법제공충분적실험의거.
In this study, 16 samples of Lily collected from different ecological sources were analyzed by RAPD using its leaf scales. The bands of DNA extracted by ameliorated CTAB. Thirty-five primers were screened from 120 random primers, and a total 769 DNA hands were amplified, 628 (81.7%) of which were polymorphic. Cluster analysis results show that the 16 Lily materials were divided into two RAPD groups at 0.940 7. There are two different genera plants: Lilium and Cardiocrinum; Lilium was divided into three different types at 0.579 0, Lilium brownii vat. viridulum, L. lancifolium and L. pumilum. These all have given good foundation for further study of Lily's molecular biology and provided the sufficient experiment basis for the new method of identifying Lily's breed strain.