中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2009年
4期
293-296
,共4页
叶永康%毕学成%韩兆冬%戴奇山%梁宇翔%蔡岳斌%何慧婵%钟惟德
葉永康%畢學成%韓兆鼕%戴奇山%樑宇翔%蔡嶽斌%何慧嬋%鐘惟德
협영강%필학성%한조동%대기산%량우상%채악빈%하혜선%종유덕
前列腺肿瘤%癌基因%逆转录聚合酶链反应%基因%HER-2%前列腺增生
前列腺腫瘤%癌基因%逆轉錄聚閤酶鏈反應%基因%HER-2%前列腺增生
전렬선종류%암기인%역전록취합매련반응%기인%HER-2%전렬선증생
Prostatic neoplasms%Oncogenes%Reverse transcriptase polymerase chain reaction%Genes%HER-2%Prostatic hyperplasia
目的 检测良性前列腺增生(BPH)与前列腺癌(PCa)组织标本HER-2及C-mvc mRNA相对值,探讨此值对PCa诊断的特异性意义.方法 通过实时荧光定量PCR检测63例PCa、37例BPH及3例正常前列腺组织HER-2及C-myc mRNA的表达,比较其在PCa与BPH中组织定量的差异.结果 相对于BPH,PCa HER-2及C-myc mRNA表达相对值分别为4.25±0.03,7.24±0.06,PCa HER-2及C-myc表达相对值较BPH高,差异有统计学意义(均为P<0.05).结论 实时荧光PCR定量检测HER-2及C-myc mRNA为PCa的诊断提供了重要的辅助指标.
目的 檢測良性前列腺增生(BPH)與前列腺癌(PCa)組織標本HER-2及C-mvc mRNA相對值,探討此值對PCa診斷的特異性意義.方法 通過實時熒光定量PCR檢測63例PCa、37例BPH及3例正常前列腺組織HER-2及C-myc mRNA的錶達,比較其在PCa與BPH中組織定量的差異.結果 相對于BPH,PCa HER-2及C-myc mRNA錶達相對值分彆為4.25±0.03,7.24±0.06,PCa HER-2及C-myc錶達相對值較BPH高,差異有統計學意義(均為P<0.05).結論 實時熒光PCR定量檢測HER-2及C-myc mRNA為PCa的診斷提供瞭重要的輔助指標.
목적 검측량성전렬선증생(BPH)여전렬선암(PCa)조직표본HER-2급C-mvc mRNA상대치,탐토차치대PCa진단적특이성의의.방법 통과실시형광정량PCR검측63례PCa、37례BPH급3례정상전렬선조직HER-2급C-myc mRNA적표체,비교기재PCa여BPH중조직정량적차이.결과 상대우BPH,PCa HER-2급C-myc mRNA표체상대치분별위4.25±0.03,7.24±0.06,PCa HER-2급C-myc표체상대치교BPH고,차이유통계학의의(균위P<0.05).결론 실시형광PCR정량검측HER-2급C-myc mRNA위PCa적진단제공료중요적보조지표.
Objective To study the HER- 2/C- myc mRNA expression in benign prostatic hyperplasia (BPH) and prostate cancer (PCa) tissue, and to explore the value of HER-2/C-myc mRNA expression in PCa diagnosis. Methods A sensitive, real-time fluorescence quantitative (FQ) PCR assay was used to detect the differential expression of HER-2 and C- myc mRNA in prostate samples from 63 patients with PCa, 37 with BPH and 3 healthy controls. Results The expression levels of HER-2 (4.25± 0.03) and C-myc (7.24±0.06) in PCa tissue were significantly higher than those in BPH (both P<0.05). Conclusion The detection of HER-2 and C-myc mRNA expression by FQ-PCR may provide reliable and helpful information for the diagnosis of PCa.
