CAJ | 학술논문

目的探讨糖尿病肾病患者外周血单核细胞(PBMC)血红素加氧酶-1(HO-1)的表达与糖尿病肾病的关系及其可能机制.方法 2型糖尿病患者伴或不伴糖尿病肾病各20例,正常对照组20例.比较各组空腹血糖、糖化血红蛋白(HbA1c)等基本资料,并检测三组血清中丙二醛(MDA)含量、外周血单核细胞活性氧(ROS)水平、HO-1 mRNA水平、HO-1蛋白表达水平.结果在正常对照组、糖尿病组、糖尿病肾病组中,血清MDA水平分别为(14.23±5.07)nmol/ml、(24.90±7.12)nmol/ml、(43.83±16.97)nmol/ml,三组相比,差异有统计学意义(F＝37.022,P＜0.01);PBMC的ROS水平分别为113.18±58.59、364.54±88.67、524.35±162.51,三组相比,差异有统计学意义(F＝68.369,P＜0.01);HO-1蛋白表达水平分别为22.84±9.98、36.72±15.85、58.1±15.93,三组相比,差异有统计学意义(F＝31.302,P＜0.01).HO-1 mRNA的水平及蛋白表达水平与MDA水平均呈正相关(r＝0.407,0.429,P＜0.05).结论糖尿病肾病患者与糖尿病非肾病患者相比处于更严重的氧化应激状态并诱导的HO-1代偿性增高.提高HO-1表达将可能是改善糖尿病肾病患者氧化应激状态的可能途径.
목적 탐토당뇨병신병환자외주혈단핵세포(PBMC)혈홍소가양매-1(HO-1)적표체여당뇨병신병적관계급기가능궤제.방법 2형당뇨병환자반혹불반당뇨병신병각20례,정상대조조20례.비교각조공복혈당、당화혈홍단백(HbA1c)등기본자료,병검측삼조혈청중병이철(MDA)함량、외주혈단핵세포활성양(ROS)수평、HO-1 mRNA수평、HO-1단백표체수평.결과 재정상대조조、당뇨병조、당뇨병신병조중,혈청MDA수평분별위(14.23±5.07)nmol/ml、(24.90±7.12)nmol/ml、(43.83±16.97)nmol/ml,삼조상비,차이유통계학의의(F＝37.022,P＜0.01);PBMC적ROS수평분별위113.18±58.59、364.54±88.67、524.35±162.51,삼조상비,차이유통계학의의(F＝68.369,P＜0.01);HO-1단백표체수평분별위22.84±9.98、36.72±15.85、58.1±15.93,삼조상비,차이유통계학의의(F＝31.302,P＜0.01).HO-1 mRNA적수평급단백표체수평여MDA수평균정정상관(r＝0.407,0.429,P＜0.05).결론 당뇨병신병환자여당뇨병비신병환자상비처우경엄중적양화응격상태병유도적HO-1대상성증고.제고HO-1표체장가능시개선당뇨병신병환자양화응격상태적가능도경.
Objective To investigate the relationship and mechanism of the heme oxygenase-1 expression in peripheral blood mononuclear cells (PBMC) and the oxidative stress in diabetic nephropathy. Methods Two groups of diabetic patients with or without diabetic nephropathy and a normal control group were enrolled in this study. Fasting blood glucose (FBG), HbA1c, serum MDA level, ROS level, HO-1 mRNA level and HO-1 protein expression in PBMC were determined. Results In control group, diabetic group and diabetic nephropathy group , the MDA levels significantly increased[(14.23±5.07)nmol/ml vs (24.90±7.12)nmol/ml vs (43.83±16.97)nmol/ml](F＝37.022,P＜0.01), the ROS levels significantly increased (113.18±58.59 vs 364.54±88.67 vs 524.35±162.51)(F＝68.369,P＜0.01) and the HO-1 protein expression also increased significantly (22.84±9.98 vs 36.72±15.85 vs 58.1±15.93)(F＝31.302,P＜0.01). There was a positive correlation among the HO-1 mRNA, protein expression and MDA level(r＝0.407,0.429,P＜0.05). Conclusions There existed a severer oxidative stress condition in patients with diabetic nephropathy compared with the patients without diabetic nephropathy. HO-1 could be a potential pathway to ameliorate oxidative stress in diabetic kidney disease patients.