CAJ | 학술논문

目的探讨核因子-κB(NF-κB)蛋白表达水平的变化与急性冠状动脉综合征(ACS)患者冠状动脉病变特征的关系.方法初步诊断为冠心病并行选择性冠状动脉造影(CAG)患者共81例.根据病情将患者分为ACS组50例,包括急性心肌梗死21例,不稳定型心绞痛29例;稳定型心绞痛(SAP)组31例;选择同期CAG正常者14例作为对照组.采用免疫印迹法检测外周血单个核细胞(PBMC)NF-κB蛋白表达水平的变化,并结合Gensini冠状动脉病变积分标准比较NF-κB蛋白表达水平与冠状动脉管腔病变程度的关系.结果 ACS组NF-κB蛋白表达水平较SAP组和对照组明显增高,分别为0.85±0.18、0.75±0.21和0.71±0.23(F=3.72,P<0.05);NF-κB蛋白表达水平与Gensini冠状动脉病变积分无相关性(r=0.07,P>O.05).结论 ACS患者存在NF-κB的激活,且NF-κB蛋白表达水平与冠状动脉病变狭窄程度无相关性.
목적 탐토핵인자-κB(NF-κB)단백표체수평적변화여급성관상동맥종합정(ACS)환자관상동맥병변특정적관계.방법 초보진단위관심병병행선택성관상동맥조영(CAG)환자공81례.근거병정장환자분위ACS조50례,포괄급성심기경사21례,불은정형심교통29례;은정형심교통(SAP)조31례;선택동기CAG정상자14례작위대조조.채용면역인적법검측외주혈단개핵세포(PBMC)NF-κB단백표체수평적변화,병결합Gensini관상동맥병변적분표준비교NF-κB단백표체수평여관상동맥관강병변정도적관계.결과 ACS조NF-κB단백표체수평교SAP조화대조조명현증고,분별위0.85±0.18、0.75±0.21화0.71±0.23(F=3.72,P<0.05);NF-κB단백표체수평여Gensini관상동맥병변적분무상관성(r=0.07,P>O.05).결론 ACS환자존재NF-κB적격활,차NF-κB단백표체수평여관상동맥병변협착정도무상관성.
Objective To explore the correlation between expression of nuclear factor kappa B (NF-kappa B) in peripheral blood monouclear cells(PBMC) and the severity of coronary artery lesion in patients with acute coronary syndrome(ACS). Methods The 81 patients were diagnosed with coronary heart disease, and all of them underwent immediate or selective coronary angiography(CAG) The nuclear protein level of activated NF-B was detected by Western blot and semi-quantity image analysis was done. Coronary angiograms were scored according to Gensini integration. The relationship between NF-κB and Gensini score was analyzed. Results The protein expression of NF-κB in PBMC in ACS group was significantly higher than that in stable angina group and control group (0.85±0.18 vs. O.75±0.21 and 0.71±0.23, F=3.72, P<0.05). The protein expression of NF-κB was not correlated with Gensini score(r=0.07, P>0.05). Conclusions The ACS patients have the activation of NF-κB in PBMC. The protein expression of NF-κB is not correlated with Gensini score, which does not suggest an implication of the severity of coronary artery.