中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2010年
1期
25-29
,共5页
翟金霞%张照祥%冯丫娟%丁书姝
翟金霞%張照祥%馮丫娟%丁書姝
적금하%장조상%풍아연%정서주
十溴联苯醚%迷宫学习%记忆%乙酰胆碱酯酶%丁酰胆碱酯酶
十溴聯苯醚%迷宮學習%記憶%乙酰膽堿酯酶%丁酰膽堿酯酶
십추련분미%미궁학습%기억%을선담감지매%정선담감지매
Deca-brominated diphenyl ether,Maze Learning%Memory%Acetylcholinesterase%Bu-tyrylcholinesterase
目的 探讨十溴联苯醚(PBDE-209)暴露对小鼠学习记忆能力的影响,为防治PBDE-209危害提供依据.方法 18只雌性BALB/c小鼠随机分成3组,每组6只,分别给予PBDE-209(1500mg·kg~(-1)·d~(-1))+精炼花生油(高剂量组)、PBDE-209(300mg·k~(-1)·d~(-1))+精炼花生油(低剂量组)和0.1 ml/10 g体重花生油(对照组)灌胃.每日1次,连续染毒4周后做Morris水迷宫试验、穿梭箱试验(期间仍然给药),测定小鼠的学习记忆能力,待染毒5周后处死.测定体重、脏器重量、脑组织中乙酰胆碱酯酶活力和丁酰胆碱酯酶活力,观察肝脏细胞组织形态学变化.结果 高剂量组心脏系数明显高于低剂量组,差异有统计学意义(P<0.05).Morris水迷宫试验结果显示,各组动物逃避潜伏期均有不同程度缩短,变化趋势差异有统计学意义(F=3.134,P<0.05);低剂量染毒组在第2象限游泳时间为(15.78±10.92)s,明显短于对照组[(28.80±8.67)s],差异有统计学意义(P<0.05).穿梭箱试验显示,各组主动逃避次数差异无统计学意义(F=3.423,P=0.06).与对照组比较,各染毒组脑组织乙酰胆碱酯酶活力和丁酰胆碱酯酶活力的差异无统计学意义(P>0.05).染毒组肝脏出现脂肪变性和肿胀,高剂量组尤为明显.结论 4周龄BALB/c小鼠暴露于PBDE-209在一定程度上可影响小鼠空间学习记忆能力.
目的 探討十溴聯苯醚(PBDE-209)暴露對小鼠學習記憶能力的影響,為防治PBDE-209危害提供依據.方法 18隻雌性BALB/c小鼠隨機分成3組,每組6隻,分彆給予PBDE-209(1500mg·kg~(-1)·d~(-1))+精煉花生油(高劑量組)、PBDE-209(300mg·k~(-1)·d~(-1))+精煉花生油(低劑量組)和0.1 ml/10 g體重花生油(對照組)灌胃.每日1次,連續染毒4週後做Morris水迷宮試驗、穿梭箱試驗(期間仍然給藥),測定小鼠的學習記憶能力,待染毒5週後處死.測定體重、髒器重量、腦組織中乙酰膽堿酯酶活力和丁酰膽堿酯酶活力,觀察肝髒細胞組織形態學變化.結果 高劑量組心髒繫數明顯高于低劑量組,差異有統計學意義(P<0.05).Morris水迷宮試驗結果顯示,各組動物逃避潛伏期均有不同程度縮短,變化趨勢差異有統計學意義(F=3.134,P<0.05);低劑量染毒組在第2象限遊泳時間為(15.78±10.92)s,明顯短于對照組[(28.80±8.67)s],差異有統計學意義(P<0.05).穿梭箱試驗顯示,各組主動逃避次數差異無統計學意義(F=3.423,P=0.06).與對照組比較,各染毒組腦組織乙酰膽堿酯酶活力和丁酰膽堿酯酶活力的差異無統計學意義(P>0.05).染毒組肝髒齣現脂肪變性和腫脹,高劑量組尤為明顯.結論 4週齡BALB/c小鼠暴露于PBDE-209在一定程度上可影響小鼠空間學習記憶能力.
목적 탐토십추련분미(PBDE-209)폭로대소서학습기억능력적영향,위방치PBDE-209위해제공의거.방법 18지자성BALB/c소서수궤분성3조,매조6지,분별급여PBDE-209(1500mg·kg~(-1)·d~(-1))+정련화생유(고제량조)、PBDE-209(300mg·k~(-1)·d~(-1))+정련화생유(저제량조)화0.1 ml/10 g체중화생유(대조조)관위.매일1차,련속염독4주후주Morris수미궁시험、천사상시험(기간잉연급약),측정소서적학습기억능력,대염독5주후처사.측정체중、장기중량、뇌조직중을선담감지매활력화정선담감지매활력,관찰간장세포조직형태학변화.결과 고제량조심장계수명현고우저제량조,차이유통계학의의(P<0.05).Morris수미궁시험결과현시,각조동물도피잠복기균유불동정도축단,변화추세차이유통계학의의(F=3.134,P<0.05);저제량염독조재제2상한유영시간위(15.78±10.92)s,명현단우대조조[(28.80±8.67)s],차이유통계학의의(P<0.05).천사상시험현시,각조주동도피차수차이무통계학의의(F=3.423,P=0.06).여대조조비교,각염독조뇌조직을선담감지매활력화정선담감지매활력적차이무통계학의의(P>0.05).염독조간장출현지방변성화종창,고제량조우위명현.결론 4주령BALB/c소서폭로우PBDE-209재일정정도상가영향소서공간학습기억능력.
Objective To investigate the effects of exposure to decabrominated diphenyl ether (PBDE-209) on learning and memory of BALB/c mice. Methods Eighteen female BALB/c mice were randomizedly divided into 3 groups and garaged with peanut oil in the control groups and 300,1500 mg·kg~(-1)·d~(-1) PBDE-209 in peanut oil daily in two exposed groups respectively for 4 weeks. The learning and memory ability of mice were tested by the Morris water maze and the shuttling box respectively. The body weight and organs index were measured and the acetyicholinesterase and butyrylcholinesterase activity in brain were determined. The liver histopathological examination was performed. Results The heart index in high dose PBDE-209 group was higher than that of the low dose PBDE-209 group(P<0.05). The results of Morris water maze showed that escape latency period was significantly shorter than the control group (F=3.134, P<0.05). The swimming time in the second quadrant of low dose PBDE-209 group was (15.78±10.92)s, significantly shorter compared with the swimming time in the second quadrant of the control group's [(28.80±8.67)s] (P<0.05). There was no significant difference in the times of active avoidance in the shuttling between three groups (F=3.423 ,P=0.06). There were no significant differences in acetylcholinesterase and butyrylcholinesterase activity in brain of PBDE-209 groups compared with the control group(P>0.05). Histologically liver damages in structure such as adipose degeneration and swelling were observed in PBDE groups. Conclusion Exposure to PBDE-209 slightly impairs the space learning and memory ability of BALB/c mice, and it has some hepototoxicity.
