CAJ | 학술논문

目的研究共刺激分子GL50-ICOS在Graves病(GD)甲状腺组织中的表达及其免疫病理意义.方法运用细胞培养、流式细胞术、RT-PCR、Western印迹和免疫组化技术,检测GD甲状腺组织和原代培养的甲状腺滤泡细胞(TFC)共刺激分子GL50和ICOS的表达.结果 (1)与正常同龄对照相比,CD4+CD28-T细胞群体在GD患者外周血中显著升高,其表面ICOS表达上调.(2)RT-PCR显示,GD患者甲状腺组织中有ICOS mRNA表达,与对照非毒性甲状腺肿(NTG)组相比具有统计学差异(P＜0.01).(3)Western印迹显示,GL50蛋白在10例GD患者组织中全部表达,较对照组差异有统计学意义(P＜0.01).(4)与对照甲状腺腺瘤组相比,GL50在20例GD患者组织切片中全部检出,而对照组无阳性表达(P＜0.01).(5)炎性细胞因子刺激体外原代培养的甲状腺滤泡细胞表面上调表达GL50(P＜0.05).结论共刺激分子GL50-ICOS在Graves病甲状腺组织异常表达.
목적 연구공자격분자GL50-ICOS재Graves병(GD)갑상선조직중적표체급기면역병리의의.방법운용세포배양、류식세포술、RT-PCR、Western인적화면역조화기술,검측GD갑상선조직화원대배양적갑상선려포세포(TFC)공자격분자GL50화ICOS적표체.결과 (1)여정상동령대조상비,CD4+CD28-T세포군체재GD환자외주혈중현저승고,기표면ICOS표체상조.(2)RT-PCR현시,GD환자갑상선조직중유ICOS mRNA표체,여대조비독성갑상선종(NTG)조상비구유통계학차이(P＜0.01).(3)Western인적현시,GL50단백재10례GD환자조직중전부표체,교대조조차이유통계학의의(P＜0.01).(4)여대조갑상선선류조상비,GL50재20례GD환자조직절편중전부검출,이대조조무양성표체(P＜0.01).(5)염성세포인자자격체외원대배양적갑상선려포세포표면상조표체GL50(P＜0.05).결론공자격분자GL50-ICOS재Graves병갑상선조직이상표체.
Objective To study the expression of co-stimulatory molecules, GL50-ICOS, in thyroid tissue of patients with Graves' disease (GD) and to explore their relationship with the immune pathogenesis of GD.Methods RT-PCR, Western blot, immunohistochemistry were applied to detect the expression of GL50-ICOS in thyroid of GD. Thyrocytes were cultured in the absence or presence of pro-inflammatory cytokines. The expression of GL50 on thyroid follicular cells (TFC) was further measured by flow cytometry. Results (1) In GD patients,the percentage of CD4+ CD28- T cells was significantly increased as compared with the control healthy individuals. The expression of co-stimulatory molecule ICOS was up-regulated. (2) The mRNA level of ICOS was significantly increased in GD patients than that in nontoxic goiter(NTG) patients(P＜0.01). (3)Compared with NTG control group, the GL50 protein expression was much higher in thyroid tissues of GD patients (P ＜0.01). (4)The results of immunohistochemistry showed that GL50 expression was observed in all GD thyroid tissues, while no expression of GL50 was detected in NTG thyroid tissues(P＜0. 01). (5) The expression of GL50on primary cultured thyroid follicular cells was significantly increased under the stimulatation of pro-inflammatory cytokines in vitro. Conclusion GL50-ICOS is expressed abnormally in thyroid tissue of patients with GD.