中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2011年
12期
990-993
,共4页
李素军%徐哲荣%鲁海峰%杨云梅
李素軍%徐哲榮%魯海峰%楊雲梅
리소군%서철영%로해봉%양운매
肠%细菌%集落计数,微生物
腸%細菌%集落計數,微生物
장%세균%집락계수,미생물
Intestinal%Bacteria%Colon count,microbial
目的 分析不同年龄健康人群肠道微生态的差异,探讨年龄与肠道菌群组成变化的相关性.方法 收集少年组、成年组和老年组健康人群的粪样,对其主要菌群进行细菌培养和计数,采用荧光定量PCR技术分析其主要菌群在属水平上的定性和定量变化,计算其反映肠道定植抗力的指标B/E值(双歧杆菌与肠杆菌数值比).结果 粪便细菌培养结果显示,成年组和老年组的双歧杆菌数量较少年组明显减少(均P＜0.01),乳酸菌数量也明显减少(分别为P＜0.01、P＜0.05)；老年组类杆菌和肠杆菌数量较少年组和成年组均显著增加(均P＜0.01)；成年组和老年组肠球菌数量较少年组明显增加(分别为P＜0.05、P＜0.01),老年组与成年组比较也明显增加(P＜0.05)；老年组的B/E值(0.88±0.13),较少年组(1.15±0.15)和成年组(1.01±0.20)显著减少(均P＜0.05).用荧光定量PCR检测双歧杆菌、类杆菌、肠杆菌数量及B/E值结果差异同细菌培养结果相符；荧光定量PCR检测少年组与老年组的乳酸菌数量差异、少年组与成年组的肠球菌数量差异较培养结果更为显著(定量PCR结果P＜0.01；培养结果P＜0.05).结论 随着年龄的增长,肠道双歧杆菌和乳酸菌数量明显减少,类杆菌、肠杆菌、肠球菌数量显著增加,肠道定植抗力也不断下降.
目的 分析不同年齡健康人群腸道微生態的差異,探討年齡與腸道菌群組成變化的相關性.方法 收集少年組、成年組和老年組健康人群的糞樣,對其主要菌群進行細菌培養和計數,採用熒光定量PCR技術分析其主要菌群在屬水平上的定性和定量變化,計算其反映腸道定植抗力的指標B/E值(雙歧桿菌與腸桿菌數值比).結果 糞便細菌培養結果顯示,成年組和老年組的雙歧桿菌數量較少年組明顯減少(均P＜0.01),乳痠菌數量也明顯減少(分彆為P＜0.01、P＜0.05)；老年組類桿菌和腸桿菌數量較少年組和成年組均顯著增加(均P＜0.01)；成年組和老年組腸毬菌數量較少年組明顯增加(分彆為P＜0.05、P＜0.01),老年組與成年組比較也明顯增加(P＜0.05)；老年組的B/E值(0.88±0.13),較少年組(1.15±0.15)和成年組(1.01±0.20)顯著減少(均P＜0.05).用熒光定量PCR檢測雙歧桿菌、類桿菌、腸桿菌數量及B/E值結果差異同細菌培養結果相符；熒光定量PCR檢測少年組與老年組的乳痠菌數量差異、少年組與成年組的腸毬菌數量差異較培養結果更為顯著(定量PCR結果P＜0.01；培養結果P＜0.05).結論 隨著年齡的增長,腸道雙歧桿菌和乳痠菌數量明顯減少,類桿菌、腸桿菌、腸毬菌數量顯著增加,腸道定植抗力也不斷下降.
목적 분석불동년령건강인군장도미생태적차이,탐토년령여장도균군조성변화적상관성.방법 수집소년조、성년조화노년조건강인군적분양,대기주요균군진행세균배양화계수,채용형광정량PCR기술분석기주요균군재속수평상적정성화정량변화,계산기반영장도정식항력적지표B/E치(쌍기간균여장간균수치비).결과 분편세균배양결과현시,성년조화노년조적쌍기간균수량교소년조명현감소(균P＜0.01),유산균수량야명현감소(분별위P＜0.01、P＜0.05)；노년조류간균화장간균수량교소년조화성년조균현저증가(균P＜0.01)；성년조화노년조장구균수량교소년조명현증가(분별위P＜0.05、P＜0.01),노년조여성년조비교야명현증가(P＜0.05)；노년조적B/E치(0.88±0.13),교소년조(1.15±0.15)화성년조(1.01±0.20)현저감소(균P＜0.05).용형광정량PCR검측쌍기간균、류간균、장간균수량급B/E치결과차이동세균배양결과상부；형광정량PCR검측소년조여노년조적유산균수량차이、소년조여성년조적장구균수량차이교배양결과경위현저(정량PCR결과P＜0.01；배양결과P＜0.05).결론 수착년령적증장,장도쌍기간균화유산균수량명현감소,류간균、장간균、장구균수량현저증가,장도정식항력야불단하강.
Objective To analyze the diversity of intestinal microecology in different healthy age groups and explore the relationship between age and the composition of intestinal microflora.Methods Fecal samples were collected from the children,adult and elderly groups.The main intestinal microflora were cultured and quantified,and the qualitative and quantitative changes in the level of category were determined by fluorescence quantitative PCR.The Bifidobacteria/Enterobacteriaceae (B/E) ratio reflexing intestinal colonization resistance was also calculated.Results The germiculture of fecal samples indicated that the counts of Bifidobacteria and Lactobacillus (P＜0.01 and P＜0.05,respectively) were significantly lower in the adult and aged groups than in children group.The number of Bacteriodes and Enterobacteriaceae in the elderly group were higher than in the children and the adult groups (all P＜0.01).The counts of Enterococcus were increased in the adult and the aged groups (P＜0.05 and P＜0.01) compared with the children group,and were higher in the aged group than in the adult group (P＜0.05).The B/E ratio was significantly decreased in the aged group (0.88-±-0.13) as compared with the children (1.15±0.15) and the adult groups ( 1.01± 0.20) ( both P ＜ 0.01).The diversities of Bifidobacteria,Bacteriodes,Enterobacteriaceae and B/E ratio by fluorescent quantification PCR were consistent with the results by germiculture technology.The diversity of Lactobacillus (P＜0.01) between the children and the aged groups as well as between the children group and the adult group significantly improved (P＜0.01 and P＜0.05 ) in PCR compared with the germiculture technology. Conclusions The numbers of intestinal Bifidobacterium and Lactobacillus are decreased with lower colonization resistance,while the numbers of Bacteriodes,Enterobacteriaceae and Enterococcus are increased with aging.
