中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2009年
1期
55-57
,共3页
祁芝珍%李超%王丽%赵海红%杨晓艳%李存香%何建%魏荣杰
祁芝珍%李超%王麗%趙海紅%楊曉豔%李存香%何建%魏榮傑
기지진%리초%왕려%조해홍%양효염%리존향%하건%위영걸
鼠疫%病原学分析%流行病学
鼠疫%病原學分析%流行病學
서역%병원학분석%류행병학
Plague%Pathogen analysis%Epidemiology
目的 研究青海省三江源地区鼠疫菌株生物学特性并确定疫源地空间结构及性质.方法 对1954-2007年青海省三江源地区分离的鼠疫菌株进行生物学表型鉴定及分子生物学研究.结果 411株代表性菌株中,12株脱氮(-)阿胶糖(-)甘油(+)属田鼠型,399株为脱氮(+)阿胶糖(+)甘油(+)属古典型.411株均能产生F1和Pst I;vw+菌株占95.13%(391/411),VW-菌株占4.87%(20/411);Pgm+菌株占80.78%(332/411)、Pgm+菌株占9%(37/411)、Pgm-菌株占10.22%(42/411).220株代表株中96.82%(213/220)的菌株对小白鼠表现为强毒株,3.18%(7,220)为中等毒力,说明绝大多数具高致病性,其毒力很强.90.02%(307/341)菌株携带6×106 45×106 x65×106质粒.80株代表株包括8个基因组型,其中6个基因型与原分型相同,另有2个新的基因组型.结论 三江源地区鼠疫菌株具备青藏高原鼠疫病原体特性,人类一旦感染可导致发病急、病情重、传染性强、病死率高等特点.
目的 研究青海省三江源地區鼠疫菌株生物學特性併確定疫源地空間結構及性質.方法 對1954-2007年青海省三江源地區分離的鼠疫菌株進行生物學錶型鑒定及分子生物學研究.結果 411株代錶性菌株中,12株脫氮(-)阿膠糖(-)甘油(+)屬田鼠型,399株為脫氮(+)阿膠糖(+)甘油(+)屬古典型.411株均能產生F1和Pst I;vw+菌株佔95.13%(391/411),VW-菌株佔4.87%(20/411);Pgm+菌株佔80.78%(332/411)、Pgm+菌株佔9%(37/411)、Pgm-菌株佔10.22%(42/411).220株代錶株中96.82%(213/220)的菌株對小白鼠錶現為彊毒株,3.18%(7,220)為中等毒力,說明絕大多數具高緻病性,其毒力很彊.90.02%(307/341)菌株攜帶6×106 45×106 x65×106質粒.80株代錶株包括8箇基因組型,其中6箇基因型與原分型相同,另有2箇新的基因組型.結論 三江源地區鼠疫菌株具備青藏高原鼠疫病原體特性,人類一旦感染可導緻髮病急、病情重、傳染性彊、病死率高等特點.
목적 연구청해성삼강원지구서역균주생물학특성병학정역원지공간결구급성질.방법 대1954-2007년청해성삼강원지구분리적서역균주진행생물학표형감정급분자생물학연구.결과 411주대표성균주중,12주탈담(-)아효당(-)감유(+)속전서형,399주위탈담(+)아효당(+)감유(+)속고전형.411주균능산생F1화Pst I;vw+균주점95.13%(391/411),VW-균주점4.87%(20/411);Pgm+균주점80.78%(332/411)、Pgm+균주점9%(37/411)、Pgm-균주점10.22%(42/411).220주대표주중96.82%(213/220)적균주대소백서표현위강독주,3.18%(7,220)위중등독력,설명절대다수구고치병성,기독력흔강.90.02%(307/341)균주휴대6×106 45×106 x65×106질립.80주대표주포괄8개기인조형,기중6개기인형여원분형상동,령유2개신적기인조형.결론 삼강원지구서역균주구비청장고원서역병원체특성,인류일단감염가도치발병급、병정중、전염성강、병사솔고등특점.
Objective To study the biological characteristics of Yersinia pestis and to develop prevention and control program on plague in Sanjiangyuan areas,Qinghai province.Methods To identify the biologic types and molecular biological features of Y.pestis isolated in Sanjiangyuan area from 1954-2007.Results Among the 411 strains ofY.pestis,12 strains belonged to the microtus type Y.pestis with denitrification (-) and donkey-bible gelatin carbohydrate(-) and glycerine(+).399 strains belonged to classic type Y.pestis with denitrification (+) and donkey-hide gelatin carbohydrate (+) and glycerine (+).411 Y.pestis strains had factor F I and Pst I.Among them,VW + strains of Y.pestis accounted for 95.13%(391/411),VW accounted for 4.87%(20/411),Pgm+accounted for 80.78%(332/411 ),Pgm±accounted for 9% (37/411 ) and Pgm-accounted for 10.22% (42/411) respectively.96.82% (213/220) of the Y.pestis strains showed strong virulence to laboratory mice while 3.18% (7/220) of the strains carried medium virulence.90.02% of the tested Y.pestis (370/411) strains had 6×106,45×106,65×106 plasmids.8 types of genome were found among 80 strains of Y.pestis,with 6 of them resembling ZHOU Dongsheng's classification.Two new genome types were found.Conclusion The Y.pestis in the Sanjiangyuan area had the characteristics of plague pathogen,identified in Qinghai-Tibet plateau.It is estimated that human beings are highly susceptible to the disease which spread fast,causing serious signs and symptoms with high death rate.
