华中科技大学学报（医学版）
華中科技大學學報（醫學版）
화중과기대학학보（의학판）
ACTA UNIVERSITATIS MEDICINAE TONGJI
2009年
6期
800-802,811
,共4页
席彦东%李双%徐钧%洪晓阳
席彥東%李雙%徐鈞%洪曉暘
석언동%리쌍%서균%홍효양
生长分化因子-5%肌腱细胞%腱鞘滑膜细胞%肌腱愈合
生長分化因子-5%肌腱細胞%腱鞘滑膜細胞%肌腱愈閤
생장분화인자-5%기건세포%건초활막세포%기건유합
growth differentiation factor-5%tendon cell%sheath cell%tendon healing
目的 观察生长分化因子-5(GDF-5)对体外培养的大鼠肌腱细胞及腱鞘滑膜细胞增殖的影响,为进一步研究在体内局部添加GDF-5以促进损伤肌腱愈合及改善外源性愈合所致粘连打下基础.方法 MTT法测定不同浓度的GDF-5在体外对肌腱及腱鞘滑膜细胞增殖率的影响;流式细胞仪测定不同浓度的GDF-5在体外对肌腱及腱鞘滑膜细胞凋亡率的影响.结果 GDF-5可以明显促进肌腱细胞的增殖,而对腱鞘滑膜细胞的增殖率的作用较弱.对两种细胞的凋亡无明显影响.结论 添加一定浓度的GDF-5后肌腱细胞增殖率较腱鞘滑膜细胞提高更为明显,而凋亡并未发生改变.
目的 觀察生長分化因子-5(GDF-5)對體外培養的大鼠肌腱細胞及腱鞘滑膜細胞增殖的影響,為進一步研究在體內跼部添加GDF-5以促進損傷肌腱愈閤及改善外源性愈閤所緻粘連打下基礎.方法 MTT法測定不同濃度的GDF-5在體外對肌腱及腱鞘滑膜細胞增殖率的影響;流式細胞儀測定不同濃度的GDF-5在體外對肌腱及腱鞘滑膜細胞凋亡率的影響.結果 GDF-5可以明顯促進肌腱細胞的增殖,而對腱鞘滑膜細胞的增殖率的作用較弱.對兩種細胞的凋亡無明顯影響.結論 添加一定濃度的GDF-5後肌腱細胞增殖率較腱鞘滑膜細胞提高更為明顯,而凋亡併未髮生改變.
목적 관찰생장분화인자-5(GDF-5)대체외배양적대서기건세포급건초활막세포증식적영향,위진일보연구재체내국부첨가GDF-5이촉진손상기건유합급개선외원성유합소치점련타하기출.방법 MTT법측정불동농도적GDF-5재체외대기건급건초활막세포증식솔적영향;류식세포의측정불동농도적GDF-5재체외대기건급건초활막세포조망솔적영향.결과 GDF-5가이명현촉진기건세포적증식,이대건초활막세포적증식솔적작용교약.대량충세포적조망무명현영향.결론 첨가일정농도적GDF-5후기건세포증식솔교건초활막세포제고경위명현,이조망병미발생개변.
Objeetive To explore the effects of growth differentiation factor-5(GDF-5) on proliferation of in vitro cultured tendon cells and sheath cells of rats,and lay the foundation for our further research on enhancing healing of hurt sheath by GDF-5 and alleviating adhesions by endogenous healing in vivo.Methods The proliferation rate of tendon cells and sheath cells was measured by using MTT assay.The apoptosis rate of tendon cells and sheath cells was detected by using flow cytometry.Results GDF-5 could obviously promote the proliferation of tendon cells,but had little impact on the proliferation of sheath cells.GDF-5 had no effects on the apoptosis of both tendon cells and sheath cells.Conclusion Addition of GDF-5 can enhance the proliferation rate of tendon cells more significantly than sheath cells.
