CAJ | 학술논문

目的: 应用气相色谱质谱仪分析前体池和产物池中同位素丰度,对清蛋白合成速率进行评估. 方法: 8只新西兰兔进行L-[~2H_5]-苯丙氨酸和肠外营养液输注,输注前和输注后每小时取静脉血3 ml.对前体池和清蛋白结合池氨基酸进行提取和衍生化后,应用气相色谱质谱仪分析两池中的同位素丰度.将同位素丰度代入计算公式,得到新西兰兔在给予肠外营养支持时的清蛋白合成率分数. 结果: 在保留281.65 s出现衍生物峰,L-[~2H_5]-苯丙氨酸衍生化产物为N,O-双三甲基硅烷基-L-苯丙氨酸.输注实验1 h后前体池同位素丰度达到稳定状态,此时清蛋白结合池同位素丰度近似于直线上升,新西兰兔在给予肠外营养时的清蛋白合成率分数为(17.26±0.92)%. 结论: 同位素示踪法是一种直接、准确、有效地测定清蛋白合成率的方法.
목적: 응용기상색보질보의분석전체지화산물지중동위소봉도,대청단백합성속솔진행평고. 방법: 8지신서란토진행L-[~2H_5]-분병안산화장외영양액수주,수주전화수주후매소시취정맥혈3 ml.대전체지화청단백결합지안기산진행제취화연생화후,응용기상색보질보의분석량지중적동위소봉도.장동위소봉도대입계산공식,득도신서란토재급여장외영양지지시적청단백합성솔분수. 결과: 재보류281.65 s출현연생물봉,L-[~2H_5]-분병안산연생화산물위N,O-쌍삼갑기규완기-L-분병안산.수주실험1 h후전체지동위소봉도체도은정상태,차시청단백결합지동위소봉도근사우직선상승,신서란토재급여장외영양시적청단백합성솔분수위(17.26±0.92)%. 결론: 동위소시종법시일충직접、준학、유효지측정청단백합성솔적방법.
Objective: To analyze the isotope enrichment in precursor and product pools by gas chromatography and mass spectrometry, and to evaluate the albumin synthesis rate. Methods: 8 New Zealand white rabbits were infused with L-[~2H_5]- phenylalanine and parenteral nutrition, 3 ml venous blood samples were extracted before and per hour after infusion. Amino acids in precursor pool and albumin-bound pool were extracted and derivatized, and isotope enrichment analysis by gas chromatography and mass spectrometry was carried out. After enrichment values were substituted into the equation, albumin fractional synthesis rate being supported with parenteral nutrition was available. Results: At the detention time 281.65 s, derivative peak showed up, and phenylalanine derivation product was N,O-Bis(trimethylsily)-L-phenylalanine according to spectrum database. Isotope enrichment in precursor pool had achieved steady state since 1 hour of infusion time passed, and simultaneously appeared to be linear increasing in albumin-bound pool. According to the enrichment values in both pools, albumin fractional synthesis rate of New Zealand white rabbits being supported with parenteral nutrition was worked out at (17.26±0.92)(n=8). Conclusion: It is direct, accurate and valid to measure albumin fractional synthesis rate by employing stable isotope.