CAJ | 학술논문

本研究从临床表现为典型血管瘤型禽白血病病例的广东某肉种鸡场的病鸡中,分离到1株J亚群禽白血病病毒(ALV-J),命名为ZH-08.利用ELISA抗原检测、PCR和间接免疫荧光试验对分离株进行鉴定,结果都呈阳性.依据ALV-J原型株HPRS-103前病毒全基因组序列设计并合成3对引物,采用分段扩增的方法完成了分离株的全基因组序列测定.结果显示该分离株基因组序列全长7 597 bp,与已公开的全基因组序列大小比较略有差异,但符合典型的复制完全型反转录病毒的基因组结构,基因序列中不含已知致癌基因.将该分离株的亚群特异性gp85基因序列与国内外各参考株相应序列进行相似性比较,发现ZH-08与YZ9901株相似性最高(93.7%).基于gp85核苷酸序列的系统进化分析表明:ZH-08株与SD07LK1株的亲缘关系最近.本研究为该毒株的生物学特性以及致病机制研究奠定了基础.
본연구종림상표현위전형혈관류형금백혈병병례적엄동모육충계장적병계중,분리도1주J아군금백혈병병독(ALV-J),명명위ZH-08.이용ELISA항원검측、PCR화간접면역형광시험대분리주진행감정,결과도정양성.의거ALV-J원형주HPRS-103전병독전기인조서렬설계병합성3대인물,채용분단확증적방법완성료분리주적전기인조서렬측정.결과현시해분리주기인조서렬전장7 597 bp,여이공개적전기인조서렬대소비교략유차이,단부합전형적복제완전형반전록병독적기인조결구,기인서렬중불함이지치암기인.장해분리주적아군특이성gp85기인서렬여국내외각삼고주상응서렬진행상사성비교,발현ZH-08여YZ9901주상사성최고(93.7%).기우gp85핵감산서렬적계통진화분석표명:ZH-08주여SD07LK1주적친연관계최근.본연구위해독주적생물학특성이급치병궤제연구전정료기출.
.A subgroup J avian leukosis virus(ALV-J), designated as ZH-08, was isolated from broiler breeder flock with a novel hemangioma case in Guangdong Province. The identified results of ELISA test, PCR and immunofluorescence assay (IFA) specific for ALV-J, were all positive.Based on the public full-length proviral genome sequence of ALV-J prototype strain HPRS-103,three pairs of primers were synthesized, and the full-length proviral genome of ZH-08 isolate was sequenced by the method of fragment amplification. The full-length proviral genome sequence of ZH-08 isolate is 7 597 bp, its size has a little difference from that of published full-length genome sequences, but its structure corresponds with typical retroviral genome structure for completely copy ability, the known oncogenes were not included in its genome. According to the gp85 sequence comparison of ZH-08 isolate with those of the other reference strains at home and abroad,the similarity with YZ9901 isolate was the highest(93.7%). Phylogenetic analysis based on gp85 gene showed that ZH-08 isolate had the closest linkage to SD07LK1 isolate. This study provide the basis of biological characteristics and pathogenesis research of ZH-08 isolate.